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Diabetic Retinopathy

Methods and Protocols

  • Book
  • © 2023

Overview

Editors:
  1. Guei-Sheung Liu

    1. Centre for Eye Research Australia, University of Melbourne, East Melbourne, Australia

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  2. Jiang-Hui Wang

    1. Centre for Eye Research Australia, University of Melbourne, East Melbourne, Australia

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2678)

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About this book

This detailed volume provides various cellular and experimental animal models and research methodologies to understand the complex interrelated pathophysiological mechanisms triggered by hyperglycemia that underlie the development of diabetic retinopathy. The book covers methods for understanding the pathogenesis of diabetic retinopathy, detection and screening of biomarkers in diabetic retinopathy, as well as novel technologies for diabetic retinopathy management. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Diabetic Retinopathy: Methods and Protocols serves as an invaluable resource for scientists and clinicians who are interested in the area of ophthalmology research.

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Keywords

Table of contents (17 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Induction of Type 2 Diabetes in Mice to Understand Vascular Changes That Drive Diabetic Retinopathy

    • Emily Attrill, Stephen M. Richards, Renee M. Ross, Brad A. Sutherland, Dino Premilovac
    Pages 1-12

  3. Akimba Proliferative Diabetic Retinopathy Model: Understanding Molecular Mechanism and Drug Screening for the Progression of Diabetic Retinopathy

    • Sai Bo Bo Tun, Veluchamy Amutha Barathi
    Pages 13-26

  4. Oxygen-Induced Retinopathy in the Rat: An Animal Model to Study the Proliferative Retinal Vascular Pathology

    • Yu-Fan Chuang, Fan-Li Lin
    Pages 27-36

  5. Retinal Assessment Using In Vivo Electroretinography and Optical Coherence Tomography in Rodent Models of Diabetes

    • Da Zhao, Pei Ying Lee, Vickie H. Y. Wong, Tomoharu Nishimura, Anh Hoang, Katie K. N. Tran et al.
    Pages 37-48

  6. A Guide to Genome-Wide Association Study Design for Diabetic Retinopathy

    • Rajya L. Gurung, Kathryn P. Burdon, Bennet J. McComish
    Pages 49-89

  7. Single-Cell RNA Sequencing (scRNA-Seq) Data Analysis of Retinal Homeostasis and Degeneration of Microglia

    • Madhu Sudhana Saddala, Srilatha Mundla, Naina Patyal, Srujanika Dash
    Pages 91-106

  8. Bioinformatics Tools for Bulk Gene Expression Deconvolution in Diabetic Retinopathy

    • Ru Qi Teh, Guei-Sheung Liu, Jiang-Hui Wang
    Pages 107-115

  9. MicroRNA Profiling from Tears as a Potential Non-invasive Method for Early Detection of Diabetic Retinopathy

    • Wilson K. M. Wong, Mya Polkamp, Ryan J. Farr, Pooja S. Kunte, Hrishikesh P. Hardikar, Chittaranjan S. Yajnik et al.
    Pages 117-134

  10. Proximity Extension Assay (PEA) Platform to Detect Vitreous Biomarkers of Diabetic Retinopathy

    • Ricardo Lamy, Showgy Ma’ayeh, Sarantis Chlamydas, Jay M. Stewart
    Pages 135-145

  11. Methods for Analyzing the Gut Microbiota in Diabetic Retinopathy

    • Yinhua Huang, Jacey Hongjie Ma, Yanfang Peng, Xu Yang, Jiansu Chen, Shibo Tang
    Pages 147-155

  12. Mass Spectrometry-Based Vitreous Proteomics: Validated Methods and Analysis Pipeline

    • Sarah Weber, Nick Carruthers, Chris Gates, Yuanjun Zhao, Jeffrey Sundstrom
    Pages 157-167

  13. Methods for Ex Vivo AAV Transduction in Rodent and Human Retinal Explants

    • Satheesh Kumar, Jiang-Hui Wang, Guei-Sheung Liu
    Pages 169-175

  14. Methods for RPE Sheet Construction Using iPS-CM in Conjunction with Natural Scaffold of Corneal Lenticule Derived from SMILE

    • Jianing Gu, Yini Wang, Chengcheng Ding, Shibo Tang, Jiansu Chen
    Pages 177-182

  15. Chromatin Immunoprecipitation Assay in Mouse Retinal Tissue

    • Zhijie Wang, Shangli Ji, Jacey Hongjie Ma, Jiansu Chen, Shibo Tang
    Pages 183-189

  16. Frozen Block Tissue Staining for Eye Structure of Zebrafish Embryo

    • Yujin Lee, Jaewook Yang
    Pages 191-198

  17. Investigating the Function of MicroRNAs in Human Retinal Microvascular Endothelial Cells of Diabetic Retinopathy

    • Yong Zeng, Zekai Cui, Jiansu Chen, Shibo Tang
    Pages 199-205

  18. Genome Editing Inhibits Retinal Angiogenesis in a Mouse Model of Oxygen-Induced Retinopathy

    • Wenyi Wu, Hetian Lei
    Pages 207-217

  19. Back Matter

    Pages 219-220
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Editors and Affiliations

  • Centre for Eye Research Australia, University of Melbourne, East Melbourne, Australia

    Guei-Sheung Liu, Jiang-Hui Wang

Bibliographic Information

  • Book Title: Diabetic Retinopathy

  • Book Subtitle: Methods and Protocols

  • Editors: Guei-Sheung Liu, Jiang-Hui Wang

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-3255-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023

  • Hardcover ISBN: 978-1-0716-3254-3Published: 17 June 2023

  • Softcover ISBN: 978-1-0716-3257-4Published: 17 June 2023

  • eBook ISBN: 978-1-0716-3255-0Published: 16 June 2023

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 220

  • Number of Illustrations: 7 b/w illustrations, 39 illustrations in colour

  • Topics: Biomedicine, general, Anatomy, Ophthalmology

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