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Nuclear DNA Content Estimation of Seaweed by Fluorimetry Analysis

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Plant Cytogenetics and Cytogenomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2672))

Abstract

Fluorimetry analysis of nuclear DNA content allows identification of genome size and ploidy levels of different life phases, tissues, and populations in seaweed species. It is an easy method that saves time and resources compared to more complex techniques. Here we describe the methodology for measuring nuclear DNA content in seaweed species by DAPI fluorochrome staining and its comparison with the standard Gallus gallus erythrocytes nuclear content, one of the preferred internal standards. With this methodology, up to a thousand nuclei can be measured in a single staining session, allowing for a quick analysis of the studied species.

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Acknowledgments

This work is dedicated to M. Antonia Ribera Siguan. Without your participation and enthusiasm, we would not have been able to open this research line in our laboratory.

Thanks are due to Dr. Donald F. Kapraun, who kindly introduced our group to this research line and taught us the technique. Also, thanks to Dr. M. Antonia Ribera Siguan, the former director of the research group “Aquatic Plant Resources” (ReVeA), funded by the Catalan government and whose contribution was essential to develop the current version of the methodology here presented. We also thank Dr. Joan Vallès Xirau, who insisted to start this research line in our group and put us into contact with Dr. Kapraun. We are also grateful to both Drs. Ribera and Vallès, as they also helped revising this work. Thanks to Dr. Elena Varela, Dr. Kjersti Sjøtun, and Dr. Franz Goecke who came to our laboratory to learn the technique and helped us to improve it. Finally, thanks are due to the editors of this book for inviting us to participate in this publication.

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Author notes

  1. Rafael P. Martín-Martín and Noemí Salvador-Soler contributed equally with all other contributors.

Authors and Affiliations

  1. Laboratori de Botànica, Facultat de Farmàcia i Ciències de l’Alimentació; Institut de Recerca de la Biodiversitat (IRBio) & Centre de Documentació de Biodiversitat Vegetal (CeDocBiV), Universitat de Barcelona, Barcelona, Spain

    Rafael P. Martín-Martín, Jordi Rull Lluch & Amelia Gómez Garreta

  2. Instituto de Ciencias Biomédicas, Universidad Autónoma de Chile, Temuco, Chile

    Noemí Salvador-Soler

Authors
  1. Rafael P. Martín-Martín
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  2. Noemí Salvador-Soler
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  3. Jordi Rull Lluch
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  4. Amelia Gómez Garreta
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Corresponding author

Correspondence to Amelia Gómez Garreta .

Editor information

Editors and Affiliations

  1. Institute of Botany, TU Dresden, Dresden, Germany

    Tony Heitkam

  2. Botanical Institute of Barcelona, Barcelona, Spain

    Sònia Garcia

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Martín-Martín, R.P., Salvador-Soler, N., Lluch, J.R., Garreta, A.G. (2023). Nuclear DNA Content Estimation of Seaweed by Fluorimetry Analysis. In: Heitkam, T., Garcia, S. (eds) Plant Cytogenetics and Cytogenomics. Methods in Molecular Biology, vol 2672. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3226-0_3

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  • DOI: https://doi.org/10.1007/978-1-0716-3226-0_3

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3225-3

  • Online ISBN: 978-1-0716-3226-0

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