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Fabrication of Protein Macromolecular Frameworks (PMFs) and Their Application in Catalytic Materials

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Protein Cages

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2671))

Abstract

The construction of three-dimensional (3D) array materials from nanoscale building blocks has drawn significant interest because of their potential to exhibit collective properties and functions arising from the interactions between individual building blocks. Protein cages such as virus-like particles (VLPs) have distinct advantages as building blocks for higher-order assemblies because they are extremely homogeneous in size and can be engineered with new functionalities by chemical and/or genetic modification. In this chapter, we describe a protocol for constructing a new class of protein-based superlattices, called protein macromolecular frameworks (PMFs). We also describe an exemplary method to evaluate the catalytic activity of enzyme-enclosed PMFs, which exhibit enhanced catalytic activity due to the preferential partitioning of charged substrates into the PMF.

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Acknowledgments

This work was supported by a grant from the Human Frontier Science Program (HFSP) 4124801. M.U. was supported in part by the National Science Foundation grant CMMI-1922883. E.S. was partially supported by the Graduate Training Program in Quantitative and Chemical Biology under Award T32 GM109825 and Indiana University. T.D. was additionally supported by the National Science Foundation through grant 1720625.

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Authors and Affiliations

  1. Department of Chemistry and Biochemistry, California State University, Fresno, Fresno, CA, USA

    Masaki Uchida & Kimberly McCoy

  2. Department of Chemistry, Indiana University, Bloomington, IN, USA

    Ekaterina Selivanovitch & Trevor Douglas

Authors
  1. Masaki Uchida
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  2. Ekaterina Selivanovitch
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  3. Kimberly McCoy
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  4. Trevor Douglas
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Corresponding author

Correspondence to Masaki Uchida .

Editor information

Editors and Affiliations

  1. Tokyo Institute Of Technology, Yokohama, Japan

    Takafumi Ueno

  2. Chemical & Biomedical Enginering, Nanyang Technological University, Singapore, Singapore

    Sierin Lim

  3. Nanyang Technological University, Singapore, Singapore

    Kelin Xia

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Uchida, M., Selivanovitch, E., McCoy, K., Douglas, T. (2023). Fabrication of Protein Macromolecular Frameworks (PMFs) and Their Application in Catalytic Materials. In: Ueno, T., Lim, S., Xia, K. (eds) Protein Cages. Methods in Molecular Biology, vol 2671. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3222-2_6

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  • DOI: https://doi.org/10.1007/978-1-0716-3222-2_6

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3221-5

  • Online ISBN: 978-1-0716-3222-2

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