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Methods and Protocols for Using Extracellular Vesicles as Delivery Vehicles in Neuronal Research

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Cell-Secreted Vesicles

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2668))

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Abstract

Extracellular vesicles (EVs) transport nucleic acids, proteins, and lipid molecules for intercellular communication. The biomolecular cargo from EVs can modify the recipient cell genetically, physiologically, and pathologically. This innate ability of EVs can be harnessed to deliver the cargo of interest to a specific organ or a cell type. Importantly, due to their ability to cross the blood–brain barrier (BBB), the EVs can be used as delivery vehicles to transport therapeutic drugs and other macromolecules to inaccessible organs such as the brain. Therefore, the current chapter includes laboratory techniques and protocols focusing on the customization of EVs for neuronal research.

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Authors and Affiliations

  1. Burnett School of Biomedical Sciences, College of Medicine, University of Central Florida, Orlando, FL, USA

    Manjusha Vaidya, Nasif Sayeed, Caroline Hobson, Sandeep Sreerama, Jonhoi Smith, Riya Shah & Kiminobu Sugaya

Authors
  1. Manjusha Vaidya
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  2. Nasif Sayeed
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  3. Caroline Hobson
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  4. Sandeep Sreerama
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  5. Jonhoi Smith
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  6. Riya Shah
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  7. Kiminobu Sugaya
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Corresponding author

Correspondence to Kiminobu Sugaya .

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Editors and Affiliations

  1. GeneCellNano, Oulu University, Oulu, Finland

    Seppo Vainio

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Vaidya, M. et al. (2023). Methods and Protocols for Using Extracellular Vesicles as Delivery Vehicles in Neuronal Research. In: Vainio, S. (eds) Cell-Secreted Vesicles. Methods in Molecular Biology, vol 2668. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3203-1_13

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  • DOI: https://doi.org/10.1007/978-1-0716-3203-1_13

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3202-4

  • Online ISBN: 978-1-0716-3203-1

  • eBook Packages: Springer Protocols

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