Abstract
CRISPR/Cas9 is the genome-editing technology that is most widely used around the world. Its widespread adoption is largely due to its simplicity and ease of use. Here, we introduce the construction of vectors and genome editing of the target gene in cells using the CRISPR/Cas9 system.
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Acknowledgments
This work was supported by grants from the Basic Science and Platform Technology Program for Innovative Biological Medicine from the Ministry of Education, Culture, Sports, Science, and Technology, Japan (MEXT); the Research Support Project for Life Science and Drug Discovery (Basis for Supporting Innovative Drug Discovery and Life Science Research (BINDS)) from Agency for Medical Research and Development (AMED) under Grant Number JP22ama121049; and the Practical Research Project for Rare/Intractable Diseases from AMED.
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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
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Hatada, I., Morita, S., Horii, T. (2023). CRISPR/Cas9. In: Hatada, I. (eds) Genome Editing in Animals. Methods in Molecular Biology, vol 2637. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3016-7_3
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DOI: https://doi.org/10.1007/978-1-0716-3016-7_3
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-3015-0
Online ISBN: 978-1-0716-3016-7
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