Abstract
The bioactive sphingolipid sphingosine 1-phosphate (S1P) and its five cognate receptors (S1PR1-5) have been implicated to play important role in multiple aspects of human physiology and diseases. The S1P-S1PR1 signaling axis in endothelial cells is crucial for establishing flow competent blood vessels. The role of S1P in neovascular pathology is of great interest and is evolving as a promising target for treatment. Here we describe an easy and affordable in vivo model of corneal neovascularization using an alkali chemical burn to the cornea. This method gives a consistent and easy-to-quantitate procedure for neovascularization and angiogenesis studies.
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Acknowledgments
NM: NIH grants EY022071, EY031316. VA Merit Review Award I01BX004893, DOD Vision Research Program Award W81XWH-20–1-0900 and Research to Prevent Blindness, USA. JLW: T32EY023202.
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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
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Wilkerson, J.L., Basu, S.K., Mandal, N.A. (2023). Angiogenesis Model of Cornea to Understand the Role of Sphingosine 1-Phosphate. In: Bhattacharya, S.K. (eds) Lipidomics. Methods in Molecular Biology, vol 2625. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2966-6_20
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DOI: https://doi.org/10.1007/978-1-0716-2966-6_20
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-2965-9
Online ISBN: 978-1-0716-2966-6
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