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Methods to Assess the Role of PARPs in Regulating Mitochondrial Oxidative Function

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Poly(ADP-Ribose) Polymerase

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2609))

Abstract

PARP enzymes are involved in metabolic regulation and impact on a plethora of cellular metabolic pathways, among them, mitochondrial oxidative metabolism. The detrimental effects of PARP1 overactivation upon oxidative stress on mitochondrial oxidative metabolism was discovered in 1998. Since then, there was an enormous blooming in the understanding of the interplay between PARPs and mitochondria. Mitochondrial activity can be assessed by a comprehensive set of methods that we aim to introduce here.

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Acknowledgments

The work was supported by grants from the NKFIH (K123975, K142141, FK128387, TKP2021-EGA-19, TKP2021-EGA-20). The Project no. TKP2021-EGA-19 and TKP2021-EGA-20 has been implemented with the support provided from the National Research, Development and Innovation Fund of Hungary, financed under the TKP2021-EGA funding scheme. The POST-COVID2021-33 grant to PB was from the Hungarian Academy of Sciences. EM is supported by the Bolyai Fellowship of the Hungarian Academy of Sciences.

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Authors and Affiliations

  1. Department of Medical Chemistry, Faculty of Medicine, University of Debrecen, Debrecen, Hungary

    Tünde Kovács, Boglárka Rauch, Edit Mikó & Péter Bai

  2. MTA-DE Lendület Laboratory of Cellular Metabolism, Debrecen, Hungary

    Péter Bai

  3. Research Center for Molecular Medicine, University of Debrecen, Debrecen, Hungary

    Péter Bai

  4. MTA-DE Cell Biology and Signaling Research Group, Debrecen, Hungary

    Péter Bai

Authors
  1. Tünde Kovács
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  2. Boglárka Rauch
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  3. Edit Mikó
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  4. Péter Bai
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Corresponding author

Correspondence to Péter Bai .

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Editors and Affiliations

  1. Department of Biomedical Sciences, University of North Dakota, Grand Forks, ND, USA

    Alexei V. Tulin

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Kovács, T., Rauch, B., Mikó, E., Bai, P. (2023). Methods to Assess the Role of PARPs in Regulating Mitochondrial Oxidative Function. In: Tulin, A.V. (eds) Poly(ADP-Ribose) Polymerase. Methods in Molecular Biology, vol 2609. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2891-1_14

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  • DOI: https://doi.org/10.1007/978-1-0716-2891-1_14

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2890-4

  • Online ISBN: 978-1-0716-2891-1

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