Abstract
The protein cargo of extracellular vesicles (EVs) determines their impact on recipient cell types and the downstream effects on biological function. Environmental cues can modify EV loading with proteins derived from the plasma membrane via endocytosis, obtained from the preexisting cytosolic pool via active sorting, or packaging with newly synthesized proteins drawn from trans-golgi networks. Given the major impact these pathways exert on EV content and functional potential, it is important to study how defined stimuli influence protein sorting into these vesicles for dispersal. To this end, pSILAC-based approaches can be used to pulse/trace the origins of EV protein content and thereby provide valuable insight into vesicle biology and likely effects on intercellular communication in diverse settings.
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Acknowledgments
This work is in part supported by the Singapore National Medical Research Council (NMRC/OFIRG/0003/2016) and Singapore Ministry of Education (MOE2018-T1-001-078).
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Ngan, SF.C., McCarthy, N.E., Sze, S.K. (2023). pSILAC-Based Determination of Cellular Protein Sorting into Extracellular Vesicles. In: Luque-Garcia, J.L. (eds) SILAC. Methods in Molecular Biology, vol 2603. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2863-8_4
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DOI: https://doi.org/10.1007/978-1-0716-2863-8_4
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