Abstract
The advent of high-throughput sequencing techniques has revolutionized biological research. One such method is RNA sequencing, which has become a relatively affordable and routine method for quantifying and comparing gene expression changes over desired experimental conditions. Along with the popularity of the method, a myriad of user-friendly, open-source computational tools have also emerged for differential gene expression analyses. Correspondingly, decades of mechanobiology research have established that mechanical cues, both alone and/or in combination with biochemical signals, can be powerful regulators of transcriptional programs and consequently cell state/fate transitions. Thus, it has become possible to investigate both universal and specific temporally resolved transcriptional responses upon mechanical stimulation genome-wide. This chapter will describe methods to analyze transcriptional changes in response to extrinsic mechanical stretch.
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Acknowledgments
This work was supported by the Intramural Research Program at the National Institutes of Health (NIH)/the National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK).
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Miroshnikova, Y.A. (2023). Monitoring Mechano-Regulation of Gene Expression by RNA Sequencing. In: Zaidel-Bar, R. (eds) Mechanobiology. Methods in Molecular Biology, vol 2600. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2851-5_19
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DOI: https://doi.org/10.1007/978-1-0716-2851-5_19
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Publisher Name: Humana, New York, NY
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Online ISBN: 978-1-0716-2851-5
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