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Bioinformatic Analysis of the Subproteomic Profile of Cardiomyopathic Tissue

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Difference Gel Electrophoresis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2596))

Abstract

Following large-scale protein separation by two-dimensional gel electrophoresis or liquid chromatography, mass spectrometry–based proteomics can be used for the swift identification and characterization of cardiac proteins and their various proteoforms. Comparative cardiac proteomics has been widely applied for the systematic analysis of heart disease and the establishment of novel diagnostic protein biomarkers. The X-linked neuromuscular disorder Duchenne muscular dystrophy is a multisystemic disease that is characterized by late-onset cardiomyopathy. This chapter outlines the bioinformatic analysis of the subproteomic profile of cardiac tissue from wild-type versus the dystrophic mdx-4cv mouse model of dystrophinopathy.

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Acknowledgments

Research in the author’s laboratory has been supported by a project grant from the Kathleen Lonsdale Institute for Human Health Research, Maynooth University and equipment funding under the Research Infrastructure Call 2012 by Science Foundation Ireland (SFI-12/RI/2346/3).

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Authors and Affiliations

  1. Charles River Laboratories, Chesterford Research Park, Saffron Walden, UK

    Sandra Murphy

  2. Department of Neonatology and Pediatric Intensive Care, Children’s Hospital, University of Bonn, Bonn, Germany

    Margit Zweyer

  3. Institute of Physiology, University of Bonn, Bonn, Germany

    Dieter Swandulla

  4. Department of Biology, Maynooth University, National University of Ireland, Maynooth, Co. Kildare, Ireland

    Kay Ohlendieck

Authors
  1. Sandra Murphy
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  2. Margit Zweyer
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  3. Dieter Swandulla
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  4. Kay Ohlendieck
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Corresponding author

Correspondence to Kay Ohlendieck .

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Editors and Affiliations

  1. Maynooth University, MAYNOOTH, Ireland

    Kay Ohlendieck

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Murphy, S., Zweyer, M., Swandulla, D., Ohlendieck, K. (2023). Bioinformatic Analysis of the Subproteomic Profile of Cardiomyopathic Tissue. In: Ohlendieck, K. (eds) Difference Gel Electrophoresis. Methods in Molecular Biology, vol 2596. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2831-7_26

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  • DOI: https://doi.org/10.1007/978-1-0716-2831-7_26

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2830-0

  • Online ISBN: 978-1-0716-2831-7

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