Abstract
Pancreatic islet transplantation is a promising cell replacement treatment for patients afflicted with type 1 diabetes (T1D), which is an autoimmune disease resulting in the destruction of insulin-producing islet β-cells. However, the shortage of donor pancreatic islets significantly hampers the widespread application of this strategy as routine therapy. Pluripotent stem cell-derived insulin-producing islet organoids constitute a promising alternative β-cell source for T1D patients. Early after transplantation, it is critical to know the fate of transplanted islet organoids, but determining their survival remains a significant technical challenge. Bioluminescence imaging (BLI) is an optical molecular imaging technique that detects the survival of living cells using light emitted from luciferase-expressing bioreporter cells. Through BLI, the post-transplantation fate of islet organoids can be evaluated over time in a noninvasive fashion with minimal intervention, thus making BLI an ideal tool to determine the success of the transplant and improving cell replacement therapy approaches for T1D.
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References
Lebastchi J, Herold KC (2012) Immunologic and metabolic biomarkers of beta-cell destruction in the diagnosis of type 1 diabetes. Cold Spring Harb Perspect Med 2(6):a007708. https://doi.org/10.1101/cshperspect.a007708
Cnop M, Welsh N, Jonas JC, Jorns A, Lenzen S, Eizirik DL (2005) Mechanisms of pancreatic beta-cell death in type 1 and type 2 diabetes: many differences, few similarities. Diabetes 54(Suppl 2):S97–S107. https://doi.org/10.2337/diabetes.54.suppl_2.s97
Brennan DC, Kopetskie HA, Sayre PH, Alejandro R, Cagliero E, Shapiro AM, Goldstein JS, DesMarais MR, Booher S, Bianchine PJ (2016) Long-term follow-up of the edmonton protocol of islet transplantation in the United States. Am J Transplant 16(2):509–517. https://doi.org/10.1111/ajt.13458
de Almeida PE, van Rappard JR, Wu JC (2011) In vivo bioluminescence for tracking cell fate and function. Am J Physiol Heart Circ Physiol 301(3):H663–H671. https://doi.org/10.1152/ajpheart.00337.2011
Kim JE, Kalimuthu S, Ahn BC (2015) In vivo cell tracking with bioluminescence imaging. Nucl Med Mol Imaging 49(1):3–10. https://doi.org/10.1007/s13139-014-0309-x
Close DM, Xu T, Sayler GS, Ripp S (2011) In vivo bioluminescent imaging (BLI): noninvasive visualization and interrogation of biological processes in living animals. Sensors (Basel) 11(1):180–206. https://doi.org/10.3390/s110100180
Marques SM, Esteves da Silva JC (2009) Firefly bioluminescence: a mechanistic approach of luciferase catalyzed reactions. IUBMB Life 61(1):6–17. https://doi.org/10.1002/iub.134
Lewis-Israeli YR, Wasserman AH, Gabalski MA, Volmert BD, Ming Y, Ball KA, Yang W, Zou J, Ni G, Pajares N, Chatzistavrou X, Li W, Zhou C, Aguirre A (2021) Self-assembling human heart organoids for the modeling of cardiac development and congenital heart disease. Nat Commun 12(1):5142. https://doi.org/10.1038/s41467-021-25329-5
Pagliuca FW, Millman JR, Gurtler M, Segel M, Van Dervort A, Ryu JH, Peterson QP, Greiner D, Melton DA (2014) Generation of functional human pancreatic beta cells in vitro. Cell 159(2):428–439. https://doi.org/10.1016/j.cell.2014.09.040
Russ HA, Parent AV, Ringler JJ, Hennings TG, Nair GG, Shveygert M, Guo T, Puri S, Haataja L, Cirulli V, Blelloch R, Szot GL, Arvan P, Hebrok M (2015) Controlled induction of human pancreatic progenitors produces functional beta-like cells in vitro. EMBO J 34(13):1759–1772. https://doi.org/10.15252/embj.201591058
Sun A, Hayat H, Liu S, Tull E, Bishop JO, Dwan BF, Gudi M, Talebloo N, Dizon JR, Li W, Gaudet J, Alessio A, Aguirre A, Wang P (2021) 3D in vivo magnetic particle imaging of human stem cell-derived islet organoid transplantation using a machine learning algorithm. Front Cell Dev Biol 9:704483. https://doi.org/10.3389/fcell.2021.704483
Szot GL, Koudria P, Bluestone JA (2007) Transplantation of pancreatic islets into the kidney capsule of diabetic mice. J Vis Exp 9:404. https://doi.org/10.3791/404
Wang P, Yigit MV, Medarova Z, Wei L, Dai G, Schuetz C, Moore A (2011) Combined small interfering RNA therapy and in vivo magnetic resonance imaging in islet transplantation. Diabetes 60(2):565–571. https://doi.org/10.2337/db10-1400
Wang P, Yigit MV, Ran C, Ross A, Wei L, Dai G, Medarova Z, Moore A (2012) A theranostic small interfering RNA nanoprobe protects pancreatic islet grafts from adoptively transferred immune rejection. Diabetes 61(12):3247–3254. https://doi.org/10.2337/db12-0441
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Sun, A., Kenyon, E., Gudi, M., Li, W., Aguirre, A., Wang, P. (2023). In Vivo Bioluminescence for the Detection of the Fate of Pancreatic Islet Organoids Post-transplantation. In: Moore, A., Wang, P. (eds) Type-1 Diabetes. Methods in Molecular Biology, vol 2592. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2807-2_14
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DOI: https://doi.org/10.1007/978-1-0716-2807-2_14
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