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Analysis of HDACi-Coupled Nanoparticles: Opportunities and Challenges

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HDAC/HAT Function Assessment and Inhibitor Development

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2589))

Abstract

Systemic administration of histone deacetylase inhibitors (HDACi), like valproic acid (VPA), is often associated with rapid drug metabolization and untargeted tissue distribution. This requires high-dose application that can lead to unintended side effects. Hence, drug carrier systems such as nanoparticles (NPs) are developed to circumvent these disadvantages by enhancing serum half-life as well as organ specificity.

This chapter gives a summary of the biological characterization of HDACi-coupled NPs in vitro, including investigation of cellular uptake, biocompatibility, as well as intracellular drug release and activity. Suitable methods, opportunities, and challenges will be discussed to provide general guidelines for the analysis of HDACi drug carrier systems with a special focus on recently developed cellulose-based VPA-coupled NPs.

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Acknowledgments

The authors acknowledge financial support by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) through the Collaborative Research Center SFB 1278 PolyTarget (Project-ID 316213987, projects A02, C02, Z01).

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Author notes

  1. Authors Marie Kühne and Susanne Hofmann have equally contributed to this chapter.

Authors and Affiliations

  1. Institute of Biochemistry and Biophysics, Center for Molecular Biomedicine, Friedrich Schiller University Jena, Jena, Germany

    Marie Kühne, Susanne Hofmann, Maren Godmann & Thorsten Heinzel

  2. Institute for Organic Chemistry and Macromolecular Chemistry, Center of Excellence for Polysaccharide Research, Friedrich Schiller University Jena, Jena, Germany

    Henry Lindemann, Andreas Koschella & Thomas Heinze

  3. Applied Systems Biology, Leibniz Institute for Natural Product Research and Infection Biology, Hans Knöll Institute, Friedrich Schiller University Jena, Jena, Germany

    Zoltán Cseresnyés & Marc Thilo Figge

  4. Division of Pharmaceutical Technology, Friedrich-Alexander-University Erlangen-Nürnberg, Erlangen, Germany

    Andreas Dzierza & Dagmar Fischer

  5. Institute of Applied Optics and Biophysics, Friedrich Schiller University Jena, Jena, Germany

    Daniel Schröder & Christian Eggeling

  6. Leibniz Institute of Photonic Technology e.V., Jena, Germany

    Christian Eggeling

  7. MRC Human Immunology Unit, Weatherall Institute of Molecular Immunology, University of Oxford, Oxford, UK

    Christian Eggeling

  8. Institute of Microbiology, Faculty of Biological Sciences, Friedrich Schiller University Jena, Jena, Germany

    Marc Thilo Figge

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  1. Marie Kühne
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  10. Dagmar Fischer
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  11. Marc Thilo Figge
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  13. Thorsten Heinzel
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Corresponding author

Correspondence to Thorsten Heinzel .

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Editors and Affiliations

  1. Institute of Toxicology, University Medical Center Mainz, Mainz, Germany

    Oliver H. Krämer

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Kühne, M. et al. (2023). Analysis of HDACi-Coupled Nanoparticles: Opportunities and Challenges. In: Krämer, O.H. (eds) HDAC/HAT Function Assessment and Inhibitor Development. Methods in Molecular Biology, vol 2589. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2788-4_9

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  • DOI: https://doi.org/10.1007/978-1-0716-2788-4_9

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2787-7

  • Online ISBN: 978-1-0716-2788-4

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