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Proximity-Dependent In Vivo Biotin Labeling for Interactome Mapping in Marchantia polymorpha

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Plant Proteostasis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2581))

Abstract

Weak or transient protein-protein interactions (PPIs) are involved in a manifold of cellular processes in all living organisms, including plants. However, many of these interactions may remain undiscovered by co-immunoprecipitation (Co-IP) approaches due to their low binding affinities or transitory nature. Enzyme-mediated proximity-dependent in vivo biotin labeling can be a powerful strategy to efficiently capture weak and transient PPIs and has been successfully applied in different model angiosperm species. Here, we provide an optimized and robust protocol for biotin ligase-mediated proximity labeling for interactome mapping in the model liverwort Marchantia polymorpha.

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Acknowledgments

We thank Rozina Kardakaris for editing the manuscript. This project was supported by the Max Planck Society and was carried out in the framework of MAdLand (http://madland.science, Deutsche Forschungsgemeinschaft (DFG) priority program 2237). H.N. is grateful for the funding by the DFG (NA 946/1-1).

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Authors and Affiliations

  1. Basic Immune System of Plants, Max-Planck Institute for Plant Breeding Research, Cologne, Germany

    Katharina Melkonian, Anne Harzen & Hirofumi Nakagami

  2. Protein Mass Spectrometry, Max-Planck Institute for Plant Breeding Research, Cologne, Germany

    Sara Christina Stolze, Anne Harzen & Hirofumi Nakagami

Authors
  1. Katharina Melkonian
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  2. Sara Christina Stolze
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  3. Anne Harzen
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  4. Hirofumi Nakagami
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Corresponding author

Correspondence to Hirofumi Nakagami .

Editor information

Editors and Affiliations

  1. Center for Research in Agricultural Genomics (CSIC-IRTA-UAB-UB), Barcelona, Spain

    L. Maria Lois

  2. Faculty of Biology, University of Freiburg, Freiburg, Germany

    Marco Trujillo

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Melkonian, K., Stolze, S.C., Harzen, A., Nakagami, H. (2023). Proximity-Dependent In Vivo Biotin Labeling for Interactome Mapping in Marchantia polymorpha. In: Lois, L.M., Trujillo, M. (eds) Plant Proteostasis. Methods in Molecular Biology, vol 2581. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2784-6_21

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  • DOI: https://doi.org/10.1007/978-1-0716-2784-6_21

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2783-9

  • Online ISBN: 978-1-0716-2784-6

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