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MRI Evaluation of Gene Therapy in the Canine Model of Duchenne Muscular Dystrophy

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Muscular Dystrophy Therapeutics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2587))

Abstract

Magnetic resonance imaging (MRI) is a well-established and widely used technique to characterize and quantify skeletal and cardiac muscle changes in Duchenne muscular dystrophy (DMD). Recently, MRI has been explored to study disease progression and response to gene therapy in the canine DMD model. Using traditional sequences, delayed gadolinium enhancement, novel sequences, and spectroscopy, investigators have begun to (i) establish the baseline MRI characteristics of the muscles in normal and affected dogs and (ii) evaluate gene therapy outcomes in treated dogs. As a noninvasive assay, MRI offers an excellent opportunity to study longitudinal muscle changes in long-term gene therapy studies in the canine model. In this chapter, we outline the MRI method used to study DMD in the canine model.

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Acknowledgments

The research on the canine DMD model in the Duan lab is currently supported by the National Institutes of Health (NS-90634 and AR-70517), Jesse Davidson Foundation-Defeat Duchenne Canada, Hope for Javier, Jackson Freel DMD Research Fund, Parent Project Muscular Dystrophy, Jett Foundation, Michael’s Cause, Ryan’s Quest, Solid Biosciences Inc., and the University of Missouri.

Disclosure

DD is a member of the scientific advisory board for Solid Biosciences and equity holders of Solid Biosciences. DD is a member of the scientific advisory board for Sardocor Corp. In the last 3 years, the Duan lab has received research supports unrelated to this project from Solid Biosciences and Edgewise.

Author information

Authors and Affiliations

  1. Department of Veterinary Medicine and Surgery, College of Veterinary Medicine, The University of Missouri, Columbia, MO, USA

    Amy R. Zalcman, Jimmy C. Lattimer, James R. Holland & John R. Dodam

  2. VetCT, Orlando, FL, USA

    Amy R. Zalcman

  3. Department of Molecular Microbiology and Immunology, School of Medicine, The University of Missouri, Columbia, MO, USA

    Chady H. Hakim & Dongsheng Duan

  4. Department of Biomedical, Biological & Chemical Engineering, College of Engineering, The University of Missouri, Columbia, MO, USA

    Dongsheng Duan

  5. Department of Neurology, School of Medicine, The University of Missouri, Columbia, MO, USA

    Dongsheng Duan

  6. Department of Biomedical Sciences, College of Veterinary Medicine, The University of Missouri, Columbia, MO, USA

    Dongsheng Duan

Authors
  1. Amy R. Zalcman
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  2. Chady H. Hakim
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  3. Jimmy C. Lattimer
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  4. James R. Holland
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  5. John R. Dodam
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  6. Dongsheng Duan
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Corresponding author

Correspondence to Dongsheng Duan .

Editor information

Editors and Affiliations

  1. Department of Medical Genetics, University of Alberta, Edmonton, AB, Canada

    Rika Maruyama

  2. Department of Medical Genetics, University of Alberta, Edmonton, AB, Canada

    Toshifumi Yokota

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Zalcman, A.R., Hakim, C.H., Lattimer, J.C., Holland, J.R., Dodam, J.R., Duan, D. (2023). MRI Evaluation of Gene Therapy in the Canine Model of Duchenne Muscular Dystrophy. In: Maruyama, R., Yokota, T. (eds) Muscular Dystrophy Therapeutics. Methods in Molecular Biology, vol 2587. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2772-3_17

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  • DOI: https://doi.org/10.1007/978-1-0716-2772-3_17

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2771-6

  • Online ISBN: 978-1-0716-2772-3

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