Abstract
The labeling of stem cells with radionuclides allows in vivo monitoring of cell migration and homing. Here, we describe the labeling of mononuclear stem cells with 99mTc and show their biodistribution in preclinical models and patients with chronic kidney disease.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Pépin M, Villain C (2020) Chronic kidney disease and cognitive impairment. Geriatr Psychol Neuropsychiatr Vieil 18:429–435. https://doi.org/10.1684/pnv.2020.0897
Centers for Disease Control and Prevention (2019) Chronic kidney disease in United States, 2019. Us Department of Health and Human Services. Centers for Disease Control and Prevention, Atlanta, GA
Ornellas FM, Ornellas DS, Martini SV et al (2017) Bone marrow–derived mononuclear cell therapy accelerates renal ischemia-reperfusion injury recovery by modulating inflammatory, antioxidant and apoptotic related molecules. Cell Physiol Biochem 41:1736–1752. https://doi.org/10.1159/000471866
Han L, Mich-Basso J, Kühn B (2021) Generation of human induced pluripotent stem cells and differentiation into cardiomyocytes. Methods Mol Biol 2158:125–139. https://doi.org/10.1007/978-1-0716-0668-1_10
Couto BG, Goldenberg RC d S, Da Fonseca LMB et al (2011) Bone marrow mononuclear cell therapy for patients with cirrhosis: a Phase 1 study. Liver Int 31:391–400. https://doi.org/10.1111/j.1478-3231.2010.02424.x
Rosado-de-Castro PH, Schmidt FR, Battistella V et al (2013) Biodistribution of bone marrow mononuclear cells after intra-arterial or intravenous transplantation in subacute stroke patients. Regen Med 8:145–155. https://doi.org/10.2217/rme.13.2
Morales MM, Souza SAL, Loivos LP et al (2015) Pilot safety study of intrabronchial instillation of bone marrow-derived mononuclear cells in patients with silicosis. BMC Pulm Med 15:1–9. https://doi.org/10.1186/s12890-015-0061-8
Escalhão CCM, Ramos IP, Hochman-Mendez C et al (2017) Safety of allogeneic canine adipose tissue-derived mesenchymal stem cell intraspinal transplantation in dogs with chronic spinal cord injury. Stem Cells Int 2017:3053759. https://doi.org/10.1155/2017/3053759
Medica D, Dellepiane S, Cantaluppi V (2020) Regenerative role of stem cell-derived extracellular vesicles in acute kidney injury. Nephron 144:638–643. https://doi.org/10.1159/000511347
Hussein EN, Hamed GM, Seif AA et al (2020) Effects of mesenchymal stem cells therapy on cardiovascular risk factors in experimental diabetic kidney disease. Can J Kidney Health Dis 7:2054358120957429. https://doi.org/10.1177/2054358120957429
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Gutfilen, B., Lopes de Souza, S.A., Morales, M.M. (2023). The Use of 99mTc-Labeled Stem Cells for Evaluation of Chronic Kidney Disease. In: Pereira, G.C. (eds) Gene, Drug, and Tissue Engineering. Methods in Molecular Biology, vol 2575. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2716-7_15
Download citation
DOI: https://doi.org/10.1007/978-1-0716-2716-7_15
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-2715-0
Online ISBN: 978-1-0716-2716-7
eBook Packages: Springer Protocols