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How to Generate a Vascular-Labelled Transgenic Zebrafish Model to Study Tumor Angiogenesis and Extravasation

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Tumor Angiogenesis Assays

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2572))

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Abstract

The use of transgenic animals carrying exogenous DNA integrated in their genome is a routine in modern-day laboratories. Nowadays, the zebrafish system represents the most useful tool for transgenesis studies mainly due to easy accessibility and manipulation of the eggs, which are produced in high numbers and over a relatively short generation time. The zebrafish transgenic technology is very straightforward when coupled with angiogenesis studies allowing easy in vivo observation of the vertebrate embryonic vasculature. Here, we describe the most common technique to generate vascular-labelled transgenic zebrafish embryos and their applications to study tumor angiogenesis and visualize tumor extravasation.

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Authors and Affiliations

  1. Laboratory of Angiogenesis and Cancer Metabolism, Department of Biology, University of Padova, Padova, Italy

    Roxana E. Oberkersch, Jacopo Lidonnici & Massimo M. Santoro

Authors
  1. Roxana E. Oberkersch
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  2. Jacopo Lidonnici
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  3. Massimo M. Santoro
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Corresponding author

Correspondence to Massimo M. Santoro .

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Editors and Affiliations

  1. Department of Basic Medical Sciences, Neurosciences and Sensory Organs, Section of Human Anatomy and Histology, University of Bari Medical School, Bari, Italy

    Domenico Ribatti

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Oberkersch, R.E., Lidonnici, J., Santoro, M.M. (2023). How to Generate a Vascular-Labelled Transgenic Zebrafish Model to Study Tumor Angiogenesis and Extravasation. In: Ribatti, D. (eds) Tumor Angiogenesis Assays. Methods in Molecular Biology, vol 2572. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2703-7_15

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  • DOI: https://doi.org/10.1007/978-1-0716-2703-7_15

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2702-0

  • Online ISBN: 978-1-0716-2703-7

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