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Isothermal Titration Calorimetry Analysis of a Cooperative Riboswitch Using an Interdependent-Sites Binding Model

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RNA Structure and Dynamics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2568))

Abstract

Isothermal titration calorimetry (ITC) is a powerful biophysical tool to characterize energetic profiles of biomacromolecular interactions without any alteration of the underlying chemical structures. In this protocol, we describe procedures for performing, analyzing, and interpreting ITC data obtained from a cooperative riboswitch–ligand interaction.

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Acknowledgments

This work was supported by NIH grants GM132185 to D.H.M and GM063162 to J.E.W. G.M.S. was supported by training grant GM118283, and C.E.C was supported by training grant AI049815 to J.E.W. and an Elon Huntington Hooker graduate fellowship.

Author information

Author notes

  1. Chapin E. Cavender and Griffin M. Schroeder contributed equally to this work.

Authors and Affiliations

  1. Department of Biochemistry & Biophysics, University of Rochester School of Medicine & Dentistry, Rochester, NY, USA

    Chapin E. Cavender, Griffin M. Schroeder, David H. Mathews & Joseph E. Wedekind

  2. Center for RNA Biology, University of Rochester School of Medicine & Dentistry, Rochester, NY, USA

    Chapin E. Cavender, Griffin M. Schroeder, David H. Mathews & Joseph E. Wedekind

Authors
  1. Chapin E. Cavender
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  2. Griffin M. Schroeder
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  3. David H. Mathews
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  4. Joseph E. Wedekind
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Corresponding authors

Correspondence to David H. Mathews or Joseph E. Wedekind .

Editor information

Editors and Affiliations

  1. Protein-Nucleic Acid Interaction Section, Center for Structural Biology, Center for Cancer Research, National Cancer Institute, Frederick, MD, USA

    Jienyu Ding

  2. Protein-Nucleic Acid Interaction Section, Center for Structural Biology, Center for Cancer Research, National Cancer Institute, Frederick, MD, USA

    Jason R. Stagno

  3. Protein-Nucleic Acid Interaction Section, Center for Structural Biology, Center for Cancer Research, National Cancer Institute, Frederick, MD, USA

    Yun-Xing Wang

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© 2023 This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply

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Cavender, C.E., Schroeder, G.M., Mathews, D.H., Wedekind, J.E. (2023). Isothermal Titration Calorimetry Analysis of a Cooperative Riboswitch Using an Interdependent-Sites Binding Model. In: Ding, J., Stagno, J.R., Wang, YX. (eds) RNA Structure and Dynamics. Methods in Molecular Biology, vol 2568. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2687-0_5

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  • DOI: https://doi.org/10.1007/978-1-0716-2687-0_5

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2686-3

  • Online ISBN: 978-1-0716-2687-0

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