Abstract
Retrogradely perfused adrenal glands have historically served for establishing many of our current knowledge on the stimulus-secretion coupling process. Although the use of intact adrenals has largely been switched to isolated chromaffin cells, adrenal glands are still a very valuable tool to characterize physiological and pharmacological questions. Even more, this is an excellent preparation for studying the splanchnic nerve/chromaffin cell interaction. In this chapter, we will provide the ways to (i) perform retrograde perfusion of isolated rat adrenals, (ii) the method to apply electrical splanchnic nerve stimulation, and (iii) the preparation of adrenals to conduct online electrochemical detection of catecholamine release.
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Acknowledgments
We are grateful to Mr. Barry Taylor (Formerly in King’s College London) for designing and building the perfusion chamber described and also to the late Dr. Pedro Michelena for conceiving the valve system. Authors are grateful to all our researchers who have been working and bringing their experience with this technique. This work was supported in part by the Spanish Ministry of Science and Innovation (grants BFU2017-82618-P and PID2020-116589GB-100 to RB).
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Borges, R., De la Iglesia, A., García, A.G. (2023). Online Detection of Catecholamine Release from the Perfused Rat Adrenal Gland. In: Borges, R. (eds) Chromaffin Cells. Methods in Molecular Biology, vol 2565. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2671-9_7
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DOI: https://doi.org/10.1007/978-1-0716-2671-9_7
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