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Vesicle Collision Protocols for the Study of Quantum Size and Exocytotic Fraction Released

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Chromaffin Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2565))

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Abstract

We review the methods of vesicle impact electrochemical cytometry, intracellular impact electrochemical cytometry, and single cell amperometry and their application to measuring the storage of neurotransmitters in cellular vesicles. We provide protocols to measure vesicle content, the release of catecholamines, and from there the fraction of transmitter released in each exocytosis event. The focus here has been a combination of methods to evaluate factors related to neuronal function at the cellular level and implications in, for example, cognition.

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Authors and Affiliations

  1. Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, Sweden

    Soodabeh Majdi, Alex S. Lima & Andrew G. Ewing

Authors
  1. Soodabeh Majdi
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  2. Alex S. Lima
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  3. Andrew G. Ewing
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Corresponding author

Correspondence to Andrew G. Ewing .

Editor information

Editors and Affiliations

  1. Department of Physical Medicine and Pharmacology, Universidad de La Laguna, La Laguna, Spain

    Ricardo Borges

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Majdi, S., Lima, A.S., Ewing, A.G. (2023). Vesicle Collision Protocols for the Study of Quantum Size and Exocytotic Fraction Released. In: Borges, R. (eds) Chromaffin Cells. Methods in Molecular Biology, vol 2565. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2671-9_16

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  • DOI: https://doi.org/10.1007/978-1-0716-2671-9_16

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2670-2

  • Online ISBN: 978-1-0716-2671-9

  • eBook Packages: Springer Protocols

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