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Growth Rate Evaluation of the Budding Yeast Saccharomyces cerevisiae Cells Carrying Endogenously Expressed Fluorescent Protein Fusions

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Fluorescent Proteins

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2564))

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Abstract

Fluorescent proteins within fluorescent fusions have been reported to affect cellular growth fitness via altering native protein function and intracellular localization. Here we report in detail a procedure to analyze the growth characteristics of yeast cells expressing such fusions in comparison to unmodified parental strain. This approach can serve as an initial step in fluorescent protein characterization in vivo.

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Acknowledgments

This work was supported by the Royal Society Newton International Fellowship Alumni (AL\191025, AL\201039), Knut and Alice Wallenberg Foundation (KAW 2017-0091, KAW 2015.0272), Swedish Research Council (VR 2019-03937), and the DAAD Rise Worldwide program (SE-BI_BI-5129).

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Authors and Affiliations

  1. Department of Microbiology and Immunology, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden

    Kara L. Schneider, Lucas E. Reibenspies, Thomas Nyström & Sviatlana Shashkova

  2. Institute of Pathology, University of Regensburg, Regensburg, Germany

    Lucas E. Reibenspies

  3. Department of Mathematical Sciences, University of Gothenburg and Chalmers University of Technology, Gothenburg, Sweden

    Sviatlana Shashkova

Authors
  1. Kara L. Schneider
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  2. Lucas E. Reibenspies
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  3. Thomas Nyström
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  4. Sviatlana Shashkova
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Corresponding author

Correspondence to Sviatlana Shashkova .

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Editors and Affiliations

  1. Institute of Molecular Plant Biology, ETH Zürich, Zürich, Switzerland

    Mayank Sharma

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Schneider, K.L., Reibenspies, L.E., Nyström, T., Shashkova, S. (2023). Growth Rate Evaluation of the Budding Yeast Saccharomyces cerevisiae Cells Carrying Endogenously Expressed Fluorescent Protein Fusions. In: Sharma, M. (eds) Fluorescent Proteins. Methods in Molecular Biology, vol 2564. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2667-2_10

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  • DOI: https://doi.org/10.1007/978-1-0716-2667-2_10

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2666-5

  • Online ISBN: 978-1-0716-2667-2

  • eBook Packages: Springer Protocols

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