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Fluorescence Correlation Spectroscopy and Phase Separation

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Phase-Separated Biomolecular Condensates

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2563))

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Abstract

A quantitative understanding of the forces controlling the assembly and functioning of biomolecular condensates requires the identification of phase boundaries at which condensates form as well as the determination of tie-lines. Here, we describe in detail how Fluorescence Correlation Spectroscopy (FCS) provides a versatile approach to estimate phase boundaries of single-component and multicomponent solutions as well as insights about the transport properties of the condensate.

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Acknowledgments

We thank Priya Banerjee, Alessandro Borgia, Jasmine Cubuk, Elliot L. Elson, Erik Martin, Tanja Mittag, Rohit Pappu, and Ben Schuler for useful discussions on phase separation and fluorescence correlation spectroscopy.

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Authors and Affiliations

  1. Department of Biochemistry and Molecular Biophysics, Washington University in St Louis, St. Louis, MO, USA

    Juan Jeremías Incicco, Debjit Roy, Melissa D. Stuchell-Brereton & Andrea Soranno

  2. Center for Science and Engineering of Living Systems (CSELS), Washington University in St Louis, St. Louis, MO, USA

    Juan Jeremías Incicco, Debjit Roy, Melissa D. Stuchell-Brereton & Andrea Soranno

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  1. Juan Jeremías Incicco
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  2. Debjit Roy
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  3. Melissa D. Stuchell-Brereton
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  4. Andrea Soranno
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Corresponding author

Correspondence to Andrea Soranno .

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Editors and Affiliations

  1. Department of Chemistry, University of Illinois at Chicago, Chicago, IL, USA

    Huan-Xiang Zhou

  2. Department of Physics, University of Illinois at Chicago, Chicago, IL, USA

    Jan-Hendrik Spille

  3. Department of Physics, University at Buffalo, State University, Buffalo, NY, USA

    Priya R. Banerjee

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Incicco, J.J., Roy, D., Stuchell-Brereton, M.D., Soranno, A. (2023). Fluorescence Correlation Spectroscopy and Phase Separation. In: Zhou, HX., Spille, JH., Banerjee, P.R. (eds) Phase-Separated Biomolecular Condensates. Methods in Molecular Biology, vol 2563. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2663-4_8

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