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Baculovirus Production and Infection in Axolotls

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Salamanders

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2562))

Abstract

Salamanders have served as an excellent model for developmental and tissue regeneration studies. While transgenic approaches are available for various salamander species, their long generation time and expensive maintenance have driven the development of alternative gene delivery methods for functional studies. We have previously developed pseudotyped baculovirus (BV) as a tool for gene delivery in the axolotl (Oliveira et al. Dev Biol 433(2):262–275, 2018). Since its initial conception, we have refined our protocol of BV production and usage in salamander models. In this chapter, we describe a detailed and versatile protocol for BV-mediated transduction in urodeles.

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Acknowledgement

The authors thank members of the PM, MHY, and EMT lab for their inputs in improving the protocol. Work in the PM laboratory is supported by grants from NIH-COBRE (5P20GM104318-08) and DFG (429469366). CRO is supported by predoctoral grant from the Portuguese Foundation for Science and Technology (SFRH/BD/51280/2010). Work in the MHY laboratory is supported by grants from DFG (22137416 & 450807335). Work in the EMT laboratory is supported by grants from ERC (AdG 742046) and FWF (Standalone I4846).

Author information

Authors and Affiliations

  1. Mount Desert Island Biological Laboratory (MDIBL), Salisbury Cove, ME, USA

    Prayag Murawala

  2. Clinic for Kidney and Hypertension Diseases, Hannover Medical School, Hannover, Germany

    Prayag Murawala

  3. Center for Regenerative Therapies (CRTD), Technische Universität Dresden, Dresden, Germany

    Catarina R. Oliveira & Maximina H. Yun

  4. Graduate Program in Areas of Basic and Applied Biology (GABBA), University of Porto, Porto, Portugal

    Catarina R. Oliveira

  5. Research – Institute of Molecular Pathology (IMP), Vienna Biocenter (VBC), Vienna, Austria

    Helena Okulski & Elly M. Tanaka

Authors
  1. Prayag Murawala
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  2. Catarina R. Oliveira
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  3. Helena Okulski
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  4. Maximina H. Yun
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  5. Elly M. Tanaka
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Corresponding authors

Correspondence to Prayag Murawala or Elly M. Tanaka .

Editor information

Editors and Affiliations

  1. Department of Biology, University of Kentucky, Lexington, KY, USA

    Ashley W. Seifert

  2. Department of Biology, Wake Forest University, Winston-Salem, NC, USA

    Joshua D. Currie

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Murawala, P., Oliveira, C.R., Okulski, H., Yun, M.H., Tanaka, E.M. (2023). Baculovirus Production and Infection in Axolotls. In: Seifert, A.W., Currie, J.D. (eds) Salamanders. Methods in Molecular Biology, vol 2562. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2659-7_24

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  • DOI: https://doi.org/10.1007/978-1-0716-2659-7_24

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2658-0

  • Online ISBN: 978-1-0716-2659-7

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