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Embryonic Tissue and Blastema Transplantations

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Salamanders

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2562))

Abstract

Embryo grafts have been an experimental pillar in developmental biology, and particularly, in amphibian biology. Grafts have been essential in constructing fate maps of different cell populations and migratory patterns. Likewise, autografts and allografts in older larvae or adult salamanders have been widely used to disentangle mechanisms of regeneration. The combination of transgenesis and grafting has widened even more the application of this technique.

In this chapter, we provide a detailed protocol for embryo transplants in the axolotl (Ambystoma mexicanum ). The location and stages to label connective tissue, muscle, or blood vessels in the limb and blood cells in the whole animal. However, the potential of embryo transplants is enormous and impossible to cover in one chapter. Furthermore, we provide a protocol for blastema transplantation as an example of allograft in older larvae.

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Acknowledgments

We are thankful to Beate Gruhl, Anja Wagner, and Dr. Judith Konantz for their dedication to the axolotls. We thank all members of the Sandoval-Guzmán Lab current and past, for their unconditional support. This work was supported by the Center for Regenerative Therapies Dresden and the German Research Council (DFG).

Author information

Authors and Affiliations

  1. Center for Regenerative Therapies Dresden (CRTD), Technische Universität Dresden, Dresden, Germany

    Maritta Schuez

  2. Center for Molecular and Cellular Bioengineering (CMCB), Technology Platform, Electron Microscopy and Histology Facility, Technische Universität Dresden, Dresden, Germany

    Thomas Kurth

  3. Department of Biology, Wake Forest University, Winston-Salem, NC, USA

    Joshua D. Currie

  4. Medical Faculty: Department of Internal Medicine 3, Center for Healthy Aging, University Hospital Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany

    Tatiana Sandoval-Guzmán

Authors
  1. Maritta Schuez
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  2. Thomas Kurth
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  3. Joshua D. Currie
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  4. Tatiana Sandoval-Guzmán
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Corresponding author

Correspondence to Tatiana Sandoval-Guzmán .

Editor information

Editors and Affiliations

  1. Department of Biology, University of Kentucky, Lexington, KY, USA

    Ashley W. Seifert

  2. Department of Biology, Wake Forest University, Winston-Salem, NC, USA

    Joshua D. Currie

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Schuez, M., Kurth, T., Currie, J.D., Sandoval-Guzmán, T. (2023). Embryonic Tissue and Blastema Transplantations. In: Seifert, A.W., Currie, J.D. (eds) Salamanders. Methods in Molecular Biology, vol 2562. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2659-7_16

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  • DOI: https://doi.org/10.1007/978-1-0716-2659-7_16

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2658-0

  • Online ISBN: 978-1-0716-2659-7

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