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Functional Analysis of Foxp3 and Its Mutants by Retroviral Transduction of Murine Primary CD4+ T Cells

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Regulatory T-Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2559))

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Abstract

The transcription factor Foxp3/FOXP3 orchestrates regulatory T (Treg) cell development and function by interacting with numerous target genes and partner proteins. Functional analysis of naturally occurring or engineered Foxp3/FOXP3 mutations has provided important insights into how the complex Foxp3/FOXP3-centered molecular network operates. Here, we describe detailed protocols for retroviral transduction of murine primary conventional CD4+ T cells to determine the impacts of Foxp3 mutations on the Treg-cell-like phenotype and function conferred by Foxp3.

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Acknowledgments

This work was supported by MEXT KAKENHI (17H06626 to R.M., 18H04025 to S.H., 19H04801 to S.H., 19 K16601 to R.M., 19 K16684 to A.N.) and by AMED PRIME (JP22gm6210018 to S.H.).

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Authors and Affiliations

  1. Laboratory of Immunology and Microbiology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo, Japan

    Akira Nakajima, Ryuichi Murakami & Shohei Hori

Authors
  1. Akira Nakajima
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  2. Ryuichi Murakami
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  3. Shohei Hori
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Corresponding author

Correspondence to Shohei Hori .

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Editors and Affiliations

  1. Imperial College London, London, UK

    Masahiro Ono

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Nakajima, A., Murakami, R., Hori, S. (2023). Functional Analysis of Foxp3 and Its Mutants by Retroviral Transduction of Murine Primary CD4+ T Cells. In: Ono, M. (eds) Regulatory T-Cells. Methods in Molecular Biology, vol 2559. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2647-4_7

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  • DOI: https://doi.org/10.1007/978-1-0716-2647-4_7

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2646-7

  • Online ISBN: 978-1-0716-2647-4

  • eBook Packages: Springer Protocols

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