Abstract
Cisternal stacking is reversible, initiated at the “cis” side of the Golgi, and gets undone at the “trans” side in a continuous cycle in tune with the cisternal maturation. TGN peeling is a hallmark of such reversible cisternal stacking, but its visualization is challenging. In wild-type cells, TGN peeling of Golgi stack happens at a lower frequency, but the event itself occurs very rapidly, making it difficult to detect by microscopy. However, we have documented that TGN peeling becomes frequent in mutants of factors that play a role in reversible cisternal stacking, such as the GRIP domain Golgin PpImh1, Arl3, or Arl1 GTPase. In the present context, we describe the quantitative live microscopic methodology to visualize the TGN peeling effect in Pichia pastoris.
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Dahara, R., Jain, B., Bhattacharyya, D. (2023). Visualizing Reversible Cisternal Stacking in Budding Yeast Pichia pastoris. In: Wang, Y., Lupashin, V.V., Graham, T.R. (eds) Golgi. Methods in Molecular Biology, vol 2557. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2639-9_28
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