Abstract
Fluorescence lifetime imaging microscopy–fluorescence resonance energy transfer (FLIM–FRET) technique is a novel tool to study protein–protein interaction combining FRET detection, lifetime measurements, and high-resolution imaging. This chapter describes a FLIM–FRET protocol for the detection and quantification of heterogeneous huntingtin aggregates in HeLa cells co-transfected with huntingtin variants containing polyglutamine tracts of different lengths.
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Lionetti, M.C., La Porta, C.A.M. (2023). FLIM-FRET Investigation of Heterogeneous Huntingtin Aggregation in HeLa Cells. In: Cieplak, A.S. (eds) Protein Aggregation. Methods in Molecular Biology, vol 2551. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2597-2_36
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DOI: https://doi.org/10.1007/978-1-0716-2597-2_36
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-2596-5
Online ISBN: 978-1-0716-2597-2
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