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Melatonin

Methods and Protocols

  • Book
  • © 2022

Overview

Editors:
  1. Ralf Jockers

    1. Inserm, U1016, Institut Cochin, PARIS, France

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Erika Cecon

    1. Inserm, U1016, Institut Cochin, PARIS, France

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains keynotes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2550)

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About this book

This volume covers the latest techniques that study the synthesis of melatonin, its receptor function, and its effects at the cellular and systemic level. The chapters are organized into three parts. Part One describes methods for the detection of melatonin and its biological derivatives in various biological samples, the manipulation of melatonin synthesis by the pineal gland in animals, and the principal source of melatonin in mammals. Part Two explores methods to measure the biological effects and consequences of melatonin binding to high-affinity G protein-coupled receptors. Part Three describes methods to measure the physiological effects that are regulated by melatonin in animals, particularly in rodent models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Melatonin: Methods and Protocols is a valuable resource for any researcher interested in investigating melatonin, from its production to its mechanisms of action and systemic effects.  



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Table of contents (47 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Methods for the Detection of Melatonin and Its Biological Derivatives

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Two-Dimensional Thin Layer Chromatography of Melatonin and Related Compounds

      • David C. Klein
      Pages 3-6

    3. Detection of Melatonin in Tissue Samples

      • Céline Legros, Jean A. Boutin
      Pages 7-12

    4. Measuring Dim Light Melatonin Onset in Humans

      • Melissa A. St. Hilaire, Steven W. Lockley
      Pages 13-20

    5. Measuring Urinary 6-Sulphatoxymelatonin in Humans

      • Melissa A. St. Hilaire, Steven W. Lockley
      Pages 21-28

    6. MEL-Index: Estimation of Tissue Melatonin Levels Using Gene Expression Data

      • Pedro Augusto C. M. Fernandes, Regina P. Markus, Gabriela Sarti Kinker
      Pages 29-32

    7. Melatonin Synthesis Enzymes Activity: Radiometric Assays for AANAT, ASMT, and TPH

      • Fernanda Gaspar do Amaral, José Cipolla-Neto, Solange Castro Afeche
      Pages 33-43

    8. Pinealectomy in Rats

      • Camila Congentino Gallo, Fernanda Akane Nishino, Fernanda Gaspar do Amaral, José Cipolla-Neto
      Pages 45-51

    9. Surgical Techniques and Nuances for Superior Cervical Ganglionectomy and Decentralization in Rats

      • Sarosh Irfan Madhani, David C. Klein, Estela M. Muñoz, Luis E. Savastano
      Pages 53-62

    10. Pineal Microdialysis

      • Caroline Aparecida Pereira de Souza, Fernanda Akane Nishino, Fernanda Gaspar do Amaral, José Cipolla-Neto
      Pages 63-74

    11. Radiochemical In Situ Hybridization in Developmental Studies of the Pineal Gland

      • Martin Fredensborg Rath, Morten Møller
      Pages 75-84

    12. Pineal Cells Dissociation and Culture: Isolated Pinealocytes, Isolated Astrocytes, and Co-culture

      • Solange Castro Afeche, Diego de Piazza Pimentel, Luís Felipe Ferro, José Cipolla-Neto
      Pages 85-94

    13. Pineal Gland Culture

      • Solange Castro Afeche, Fernanda Gaspar do Amaral, José Cipolla-Neto
      Pages 95-100

    14. Preparation of Single Pineal Cells

      • Steven L. Coon, David C. Klein
      Pages 101-104

    15. RNA Sequencing of Single Pineal Cells

      • Steven L. Coon, David C. Klein
      Pages 105-112

    16. siRNA-Mediated Downregulation of Gene Expression in Cultured Rat Pineal Cells

      • Aurea Susana Blancas-Velazquez, Martin Fredensborg Rath
      Pages 113-121

    17. Analysis of the Human Pineal Proteome by Mass Spectrometry

      • Mariette Matondo, Guillaume Dumas, Erik Maronde
      Pages 123-132

    18. The Pineal Transcriptome Webpage

      • David C. Klein, Steven L. Coon
      Pages 133-137

  3. Methods to Measure Melatonin Effects at the Molecular and Cellular Level

    1. Front Matter

      Pages 139-139
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Editors and Affiliations

  • Inserm, U1016, Institut Cochin, PARIS, France

    Ralf Jockers, Erika Cecon

Bibliographic Information

  • Book Title: Melatonin

  • Book Subtitle: Methods and Protocols

  • Editors: Ralf Jockers, Erika Cecon

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2593-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-2592-7Published: 01 October 2022

  • Softcover ISBN: 978-1-0716-2595-8Published: 02 October 2023

  • eBook ISBN: 978-1-0716-2593-4Published: 30 September 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 500

  • Number of Illustrations: 34 b/w illustrations, 101 illustrations in colour

  • Topics: Physiology, Neurosciences

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