Abstract
In this chapter, we describe a DEL “recipe” approach to hit confirmation using on-DNA cleavable linkers which can be followed by direct AS-MS evaluation and identification of binder(s) from the released small-molecule mixture. When coupled with automation, this platform creates opportunity for enabling an even higher-throughput binder confirmation (HTBC) platform to allow full interrogation of selections results. Two on-DNA cleavable strategies of releasing small molecules from DNA are reported herein.
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Xia, B., Summerfield, J.D. (2022). High-Throughput Binder Confirmation (HTBC). In: Israel, D., Ding, Y. (eds) DNA-Encoded Chemical Libraries. Methods in Molecular Biology, vol 2541. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2545-3_24
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DOI: https://doi.org/10.1007/978-1-0716-2545-3_24
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Publisher Name: Humana, New York, NY
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Online ISBN: 978-1-0716-2545-3
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