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Tracing and Manipulating Drosophila Cell Lineages Based on CRISPR: CaSSA and CLADES

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Drosophila

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2540))

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Abstract

Cell lineage defines the mitotic connection between cells that make up an organism. Mapping these connections in relation to cell identity offers an extraordinary insight into the mechanisms underlying normal and pathological development. The analysis of molecular determinants involved in the acquisition of cell identity requires gaining experimental access to precise parts of cell lineages. Recently, we have developed CaSSA and CLADES, a new technology based on CRISPR that allows targeting and labeling specific lineage branches. Here we discuss how to better exploit this technology for lineage studies in Drosophila, with an emphasis on neuronal specification.

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Author information

Author notes

  1. Jorge Garcia-Marques and Tzumin Lee contributed equally with all other contributors.

Authors and Affiliations

  1. Centro Nacional de Biotecnologia, Consejo Superior de Investigaciones Cientificas, Madrid, Spain

    Jorge Garcia-Marques

  2. Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA

    Tzumin Lee

Authors
  1. Jorge Garcia-Marques
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  2. Tzumin Lee
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Corresponding authors

Correspondence to Jorge Garcia-Marques or Tzumin Lee .

Editor information

Editors and Affiliations

  1. Institute of Genetics, Technische Universität Dresden, Dresden, Germany

    Christian Dahmann

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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Garcia-Marques, J., Lee, T. (2022). Tracing and Manipulating Drosophila Cell Lineages Based on CRISPR: CaSSA and CLADES. In: Dahmann, C. (eds) Drosophila. Methods in Molecular Biology, vol 2540. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2541-5_9

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  • DOI: https://doi.org/10.1007/978-1-0716-2541-5_9

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2540-8

  • Online ISBN: 978-1-0716-2541-5

  • eBook Packages: Springer Protocols

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