Abstract
DNA extraction from plant samples is very important for a good performance of diagnostic molecular assays in phytopathology. The variety of matrices (such as leaves, roots, and twigs) requires a differentiated approach to DNA extraction. Here we describe three categories of matrices: (a) symptomatic bark/wood tissue; (b) residues of frass resulting from insect woody trophic activities, portions of the galleries produced in the wood, and tissues surrounding exit holes; and (c) leaves of different plant species. To improve the performances of diagnostic assays, we here describe DNA extraction procedures that have been optimized for each matrix type.
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Rizzo, D. et al. (2022). DNA Extraction Methods to Obtain High DNA Quality from Different Plant Tissues. In: Luchi, N. (eds) Plant Pathology. Methods in Molecular Biology, vol 2536. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2517-0_5
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DOI: https://doi.org/10.1007/978-1-0716-2517-0_5
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Publisher Name: Humana, New York, NY
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Online ISBN: 978-1-0716-2517-0
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