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CE Coupled to ICP-MS and Single Particle ICP-MS for Nanoparticle Analysis

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Capillary Electrophoresis-Mass Spectrometry

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2531))

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Abstract

Capillary electrophoresis (CE) can be used for the separation of nanoparticles (NPs). Coupling of CE to inductively coupled plasma mass spectrometry (ICP-MS) or single particle (sp)-ICP-MS enhances the analytical performance and capabilities of the method compared to CE with a standard detector (ultraviolet visible spectroscopy), in particular for trace analysis of metals or metal-containing compounds. spICP-MS is a method for NP analysis, where a standard ICP-MS setup is used with fast time-resolved detection in order to obtain information on individual NPs. Here we describe a method for the separation and detection of silver and gold NPs using CE-ICP-MS and CE-spICP-MS with reversed electrode polarity stacking mode (REPSM) for online preconcentration. CE-spICP-MS allows obtaining the average size, size distribution, elemental composition, and particle number concentration (PNC) of NPs in addition to a CE separation profile in a single run. Moreover, CE-spICP-MS can be used in some cases to separate NPs with different coatings.

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Author information

Author notes

  1. Darya Mozhayeva

    Present address: BASF SE, Ludwigshafen, Germany

Authors and Affiliations

  1. Department of Chemistry and Biology, University of Siegen, Siegen, Germany

    Darya Mozhayeva & Carsten Engelhard

  2. Research Center of Micro- and Nanochemistry and (Bio)Technology, University of Siegen, Siegen, Germany

    Carsten Engelhard

Authors
  1. Darya Mozhayeva
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  2. Carsten Engelhard
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Corresponding author

Correspondence to Carsten Engelhard .

Editor information

Editors and Affiliations

  1. Aalen University, Aalen, Baden-Württemberg, Germany

    Christian Neusüß

  2. Aalen University, Aalen, Baden-Württemberg, Germany

    Kevin Jooß

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Mozhayeva, D., Engelhard, C. (2022). CE Coupled to ICP-MS and Single Particle ICP-MS for Nanoparticle Analysis. In: Neusüß, C., Jooß, K. (eds) Capillary Electrophoresis-Mass Spectrometry . Methods in Molecular Biology, vol 2531. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2493-7_16

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  • DOI: https://doi.org/10.1007/978-1-0716-2493-7_16

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2492-0

  • Online ISBN: 978-1-0716-2493-7

  • eBook Packages: Springer Protocols

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