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Advances in Somatic Embryogenesis in Vanilla (Vanilla planifolia Jacks.)

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Somatic Embryogenesis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2527))

Abstract

Somatic embryogenesis is an in vitro plant morphogenetic process due to cell totipotentiality to induce shoot regeneration. To induce this proliferation pathway, we used auxins such as 2,4-dichlorophenoxyacetic acid in combination with cytokinins. There are numerous somatic embryogenesis protocols for a great diversity of plants, including orchids, but none has been yet reported in Vanilla planifolia. Vanilla (V. planifolia) is propagated mainly asexually through cuttings. Under in vitro conditions, it is propagated asexually through direct and indirect organogenesis involving the use of various plant growth regulators in different concentrations. The cell response depends on explant type, culture medium used, and incubation conditions. Direct organogenesis involves de novo formation from differentiated cells; the indirect pathway develops from cell dedifferentiation that produces an explant called “callus.” In most cases, this type of cell regeneration uses Benzyladenine. The explants most used in this pathway are shoots, roots, and protocorms, although some studies report the use of other types of explants, including leaves and seeds. Somatic embryogenesis in vanilla has been poorly studied partly because of the recalcitrance of this species. This work mentioned the advances in the in vitro morphogenesis of V. planifolia, mentioning the advantages and disadvantages of each morphogenetic pathway and its characteristics.

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Authors and Affiliations

  1. Facultad de Ciencias Biológicas y Agropecuarias, Universidad Veracruzana, Amatlán de los Reyes, Veracruz, Mexico

    Marco A. Ramírez-Mosqueda, Arturo A. Armas-Silva, Marco V. Rodríguez-Deméneghi & Eduardo Martínez-Santos

  2. CONACYT-Colegio de Postgraduados-Campus Córdoba, Carretera Federal Córdoba-Veracruz km 348, Amatlán de los Reyes, Veracruz, Mexico

    Jericó J. Bello-Bello

Authors
  1. Marco A. Ramírez-Mosqueda
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  2. Jericó J. Bello-Bello
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  3. Arturo A. Armas-Silva
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  4. Marco V. Rodríguez-Deméneghi
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  5. Eduardo Martínez-Santos
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Correspondence to Marco A. Ramírez-Mosqueda .

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Editors and Affiliations

  1. Laboratorio de Micropropagación Vegetal, Facultad de Ciencias Biológicas y Agropecuarias, Universidad Veracruzana, Veracruz, Mexico

    Marco A. Ramírez-Mosqueda

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Ramírez-Mosqueda, M.A., Bello-Bello, J.J., Armas-Silva, A.A., Rodríguez-Deméneghi, M.V., Martínez-Santos, E. (2022). Advances in Somatic Embryogenesis in Vanilla (Vanilla planifolia Jacks.). In: Ramírez-Mosqueda, M.A. (eds) Somatic Embryogenesis. Methods in Molecular Biology, vol 2527. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2485-2_3

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  • DOI: https://doi.org/10.1007/978-1-0716-2485-2_3

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2484-5

  • Online ISBN: 978-1-0716-2485-2

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