Abstract
The global floriculture market is expected to reach US$41.1 billion by 2027 at a CAGR of 5% over the analysis period 2020–2027; on the year 2020, the recorded market value in this trade was US$29.2 billion. The florists mainly use Anthurium andraeanum flowers in fashionable bouquets and floral arrangements because of their beautiful, attractive bright colored eye-catching spathe, candle-like spadix, prolonged vase life, etc. The cut flower industry always seeks elite cultivars and new hybrids of A. andraeanum, that in turn depend on the availability of large numbers of clonal planting propagules. In vitro somatic embryogenesis is an important technique for large-scale clonal propagation, development of transgenic plants, creation of new variety by somaclonal variation, mutagenesis on in vitro plants, and germplasm preservation for future use. Here, we describe the protocol of somatic embryogenesis of Anthurium andraeanum cv. Cancan, an important commercial cultivated variety. The protocol has been optimized by using 4 different types of culture media which are used during embryogenic callus induction, multiplication of callus, induction of somatic embryogenesis, and maturation plus conversion of embryos into plantlets. The protocol outlines the detailed methods from mother plant procurement to hardening of micro plants that is ready to transfer in the field and it can be used for large-scale commercial propagation.
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Acknowledgments
This work was financially supported by UGC-DAE CSR, Kolkata Centre, Govt. of India (UGC-DAE-CSR-KC/CRS/19/RB-08/1043/1065 dated 10.05.2019); WBHESTB, Govt. of West Bengal [846 (Sanc.)/ST/P/S and T/2G-11/2018; 19.06.2019]; DST-PURSE PROGRAMME, GOI; and Personal Research Grants (PRG) for teachers and URS provided by the University of Kalyani, West Bengal, India.
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Bandyopadhyay, T.K. et al. (2022). Somatic Embryogenesis of Anthurium andraeanum Linden., -A Tropical Florists' Plant. In: Ramírez-Mosqueda, M.A. (eds) Somatic Embryogenesis. Methods in Molecular Biology, vol 2527. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2485-2_13
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DOI: https://doi.org/10.1007/978-1-0716-2485-2_13
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