Abstract
Bioluminescence (BL), the emission light resulting from the enzyme-catalyzed oxidative reaction, is a powerful imaging modality for monitoring biological phenomena both in vitro and in vivo. Coelenterazine (CTZ), the known widespread luciferin found in bioluminescent organisms, develops bioluminescence imaging (BLI). Here, we describe an approach to synthesize a series of novel CTZ derivatives for diversifying the toolbox of the BL substrates. Furthermore, we exemplify some of them display excellent BL signals in vitro and in vivo, and thus should be noted as one of the ideal substrates for in vivo BLI compared with a well-known conventional substrate, DeepBlueC.
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Acknowledgments
This work was supported by grants from the National Natural Science Foundation of China (81874308), the Shandong Natural Science Foundation (ZR2018ZC0233), the Taishan Scholar Program at Shandong Province, the China Postdoctoral Science Foundation (2018M642674), and the Guangdong Basic and Applied Basic Research Foundation (2020A1515110284).
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Jiang, T., Li, M. (2022). Synthetic Coelenterazine Derivatives and Their Application for Bioluminescence Imaging. In: Kim, SB. (eds) Bioluminescence. Methods in Molecular Biology, vol 2524. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2453-1_2
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DOI: https://doi.org/10.1007/978-1-0716-2453-1_2
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