Abstract
The proper maintenance of cancer cell lines is critical to maintain the integrity of any experiment. Proper maintenance includes handling of cell lines and maintaining records on usage and confirmation of identity. Cross-contamination of cancer cell lines has been well documented by the ATCC and must be avoided at all costs. In addition, contamination by Mycoplasma sp. is a well-known issue, which, if left unchecked, can affect all data collected as this contamination can affect cell line metabolism and has uncharacterized effects. Considerations and specific recommendations for the proper maintenance of cancer cell lines are presented here.
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Acknowledgments
SLC is a senior scientist with the Beatrice Hunter Cancer Research Institute. This work was funded by the Natural Sciences and Engineering Research Council of Canada and the Janeway Children’s Hospital Foundation.
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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
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Christian, S.L. (2022). Overview of the Maintenance of Authentic Cancer Cell Lines. In: Christian, S.L. (eds) Cancer Cell Biology. Methods in Molecular Biology, vol 2508. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2376-3_1
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DOI: https://doi.org/10.1007/978-1-0716-2376-3_1
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Publisher Name: Humana, New York, NY
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Online ISBN: 978-1-0716-2376-3
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