Abstract
Optogenetics is of key importance for progress in basic neuroscience research and the development of innovative future medical treatments. In particular, the use of microbial rhodopsins enables remote control of excitable-cell activity by light. The electrophysiological characterization of microbial rhodopsins is inevitable for the development of variants, which further advance optogenetic applications. Therefore, we provide a detailed description of the application of the patch-clamp method for the electrophysiological characterization of microbial rhodopsins. Here we describe the investigation of light sensitivity, wavelength- and voltage-dependence, photocurrent inactivation, kinetics, and ion selectivity.
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Acknowledgments
This work was supported by the Cluster of Excellence “Multiscale Bioimaging: from Molecular Machines to Networks of Excitable Cells” (MBExC), University of Göttingen, Germany.
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Mager, T. (2022). Electrophysiological Characterization of Microbial Rhodopsins by Patch-Clamp Experiments. In: Gordeliy, V. (eds) Rhodopsin. Methods in Molecular Biology, vol 2501. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2329-9_13
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DOI: https://doi.org/10.1007/978-1-0716-2329-9_13
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