Abstract
Creating mouse models of human genetic disease (Gurumurthy and Lloyd, Dis Models Mech 12(1):dmm029462, 2019) and livestock trait (Schering et al. Arch Physiol Biochem 121(5):194–205, 2015; Habiela et al. J Gen Virol 95 (Pt 11):2329–2345, 2014) have been proven to be a useful tool for understanding the mechanism behind the phenotypes and fundamental and applied research in livestock. A single base pair deletion of prolactin receptor (PRLR) has an impact on hair morphology phenotypes beyond its classical roles in lactation in cattle, the so-called slick cattle (Littlejohn et al. Nat Commun 5:5861, 2014). Here, we generate a knock-in mouse model by targeting the specific locus of PRLR gene using Cas9-mediated genome editing via homology-directed repair (HDR) in mouse zygotes. The mouse model carrying the identical PRLR mutation in slick cattle may provide a useful animal model to study the pathway of thermoregulation and the mechanism of heat-tolerance in the livestock.
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References
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Acknowledgment
This work was supported by Livestock Improvement Corporation Limited (LIC), New Zealand (grant code: LICNZ-CA14). The pX330 plasmid was a gift from Feng Zhang (Addgene plasmid # 42230; http://n2t.net/addgene:42230; RRID: Addgene_42230).
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Liu, J., Verma, P.J. (2022). Generating a Heat-Tolerance Mouse Model. In: Verma, P.J., Sumer, H., Liu, J. (eds) Applications of Genome Modulation and Editing. Methods in Molecular Biology, vol 2495. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2301-5_14
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DOI: https://doi.org/10.1007/978-1-0716-2301-5_14
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