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Environmental Responses in Plants

Methods and Protocols

  • Book
  • © 2022
  • Latest edition

Overview

Editors:
  1. Paula Duque

    1. Plant Molecular Biology, Instituto Gulbenkian de Ciência, Oeiras, Portugal

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    You can also search for this editor in PubMed   Google Scholar

  2. Dóra Szakonyi

    1. Plant Molecular Biology, Instituto Gulbenkian de Ciência, Oeiras, Portugal

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2494)

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Table of contents (24 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Tropisms and Other Developmental Responses

    1. Front Matter

      Pages 1-1
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    2. Analysis of Plant Root Gravitropism

      • Richard Barker, Sarah Johns, Ralph Trane, Simon Gilroy
      Pages 3-16

    3. Hydrotropism: Analysis of the Root Response to a Moisture Gradient

      • Irene Garcia-Maquilon, Jorge Lozano-Juste, Abdulwahed F. Alrefaei, Pedro L. Rodriguez
      Pages 17-24

    4. Evaluating Root Mechanosensing Response in Rice

      • Tiago F. Lourenço, André M. Cordeiro, João Frazão, Nelson J. M. Saibo, M. Margarida Oliveira
      Pages 25-35

    5. Physiological Analysis of Phototropic Responses to Blue and Red Light in Arabidopsis

      • Mathias Zeidler
      Pages 37-45

    6. Shoot Branching Phenotyping in Arabidopsis and Tomato

      • Ana Confraria, Aitor Muñoz-Gasca, Liliana Ferreira, Elena Baena-González, Pilar Cubas
      Pages 47-59

    7. A Ribo-Seq Method to Study Genome-Wide Translational Regulation in Plants

      • Hao Chen, Jose M. Alonso, Anna N. Stepanova
      Pages 61-98

  3. Photoperiodism and Circadian Rythms

    1. Front Matter

      Pages 99-99
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    2. Assessing Flowering Time Under Different Photoperiods

      • Jesús Praena, Elizabeth van Veen, Rossana Henriques, Reyes Benlloch
      Pages 101-115

    3. Analysis of Phytochrome-Dependent Seed Germination in Arabidopsis

      • Mathias Zeidler
      Pages 117-124

    4. Rhythmic Leaf and Cotyledon Movement Analysis

      • Ping Lou, Kathleen Greenham, C. Robertson McClung
      Pages 125-134

    5. Assessing Plant Pigment Regulation in Circadian Experiments

      • Raquel Esteban, Víctor Resco de Dios, José Ignacio García-Plazaola
      Pages 135-148

    6. Chemical Perturbation of Chloroplast Ca2+ Dynamics in Arabidopsis thaliana Suspension Cell Cultures and Seedlings

      • Panfeng Yao, Steffen Vanneste, Lorella Navazio, Frank Van Breusegem, Simon Stael
      Pages 149-158

  4. Abiotic Stress Responses

    1. Front Matter

      Pages 159-159
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    2. Screening for Abiotic Stress Response in Rice

      • Telma Fernandes, Fredilson Melo, Maria Beatriz Vieira, Tiago F. Lourenço, Chiara Pucciariello, Nelson J. M. Saibo et al.
      Pages 161-194

    3. Etiolated Hypocotyls: A New System to Study the Impact of Abiotic Stress on Cell Expansion

      • Guiomar Martín, Paula Duque
      Pages 195-205

    4. Assessing Postgermination Development in Arabidopsis thaliana Under Abiotic Stress

      • Rui Albuquerque-Martins, Alba Rodríguez Díez, Dóra Szakonyi, Paula Duque
      Pages 207-215

    5. Assessing Abscisic Acid-Mediated Changes in Stomatal Aperture Through High-Quality Leaf Impressions

      • Alba Rodríguez Díez, Paula Duque, Rossana Henriques
      Pages 217-227

    6. Evaluation of the Anti-transpirant Activity of ABA Receptor Agonists in Monocot and Eudicot Plants

      • Daniel Franco-Aragón, Irene García-Maquilón, Alfredo Manicardi, Pedro L. Rodríguez, Jorge Lozano-Juste
      Pages 229-238
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Keywords

About this book

This second edition describes up-to-date methodological approaches, ranging from physiological assays to imaging and molecular techniques, to study a wide variety of plant responses to environmental cues. Chapters are divided into four sections detailing protocols to investigate key biological processes underlying plant environmental responses in the model organisms Arabidopsis thaliana and Physcomitrella patens as well as in different crop species. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

 

Authoritative and cutting-edge, Environmental Responses in Plants, Second Edition aims to be a foundation for future studies and to be a source of inspirationfor new investigations in the field.  

Editors and Affiliations

  • Plant Molecular Biology, Instituto Gulbenkian de Ciência, Oeiras, Portugal

    Paula Duque, Dóra Szakonyi

Bibliographic Information

  • Book Title: Environmental Responses in Plants

  • Book Subtitle: Methods and Protocols

  • Editors: Paula Duque, Dóra Szakonyi

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2297-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-2296-4Published: 26 April 2022

  • Softcover ISBN: 978-1-0716-2299-5Published: 26 April 2023

  • eBook ISBN: 978-1-0716-2297-1Published: 25 April 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XIV, 349

  • Number of Illustrations: 10 b/w illustrations, 63 illustrations in colour

  • Topics: Plant Sciences

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