Abstract
Immunofluorescence labeling enables the detection and characterization of various parasite proteins presented on the surface of the infected red blood cell. Several approaches for immunofluorescence detection of red blood cell surface-presented proteins of Plasmodium spp. have been successfully established and published over the years. However, finding the right approach depends on the scientific question, and different protocols have different advantages. Here, we discuss some aspects that should be considered and present an easily applicable protocol for labeling parasite surface antigens, which subsequently can be analyzed by immunofluorescence microscopy (or flow cytometry).
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Acknowledgments
We would like to acknowledge the Baden-Württemberg Stiftung for funding to M.G. and the German Research Foundation (DFG) for funding to J.G. (349355339). M.G. is member of the SFB 1129. The authors further express gratitude to Patrick Pitoniak for critically proofreading this manuscript.
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Ganter, M., Guizetti, J., Kilian, N. (2022). Visualization of Infected Red Blood Cell Surface Antigens by Fluorescence Microscopy. In: Jensen, A.T.R., Hviid, L. (eds) Malaria Immunology. Methods in Molecular Biology, vol 2470. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2189-9_31
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DOI: https://doi.org/10.1007/978-1-0716-2189-9_31
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