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Microtubules

Methods and Protocols

  • Book
  • © 2022

Overview

Editors:
  1. Hiroshi Inaba

    1. Dept Chemistry and Biotechnology, Tottori University, Tottori, Japan

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2430)

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About this book

This volume provides state-of-the-art methodology to functionalize microtubules and construct microtubule-based materials. Chapters consists of six sections detailing modification of microtubule structures, observation and control of microtubule movement, material applications of microtubules in vitro, development of microtubule-binding molecules, comprehensive approaches to analyze properties of microtubules, and functionalization of microtubules in living cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Microtubules: Methods and Protocols aims to provide useful information for construction of microtubule-based materials and help to create new ideas for the next-generation of materials and applications through detailed protocols.

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Keywords

Table of contents (29 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Preparation and Modification of Microtubules

    1. Front Matter

      Pages 1-1
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    2. Purification of Tubulin from Porcine Brain and its Fluorescence Dye Modification

      • Satsuki Ishii, Mousumi Akter, Jakia Jannat Keya, Mst. Rubaya Rashid, Farhana Afroze, Syeda Rubaiya Nasrin et al.
      Pages 3-16

    3. Facile Method of Tubulin Purification from Goat Brain for Reconstitution of Microtubule-Associated Intracellular Function

      • Satyajit Ghosh, Shubham Garg, Nabanita Mukherjee, Surajit Ghosh
      Pages 17-45

    4. Functionalization of Tubulin: Approaches to Modify Tubulin with Biotin and DNA

      • Mousumi Akter, Jakia Jannat Keya, Arif Md. Rashedul Kabir, Mst. Rubaya Rashid, Satsuki Ishii, Akira Kakugo
      Pages 47-59

    5. Electro-Modulation of Tubulin Properties and Function

      • Djamel Eddine Chafai, Michal Cifra
      Pages 61-70

  3. Observation and Control of Microtubule Movement

    1. Front Matter

      Pages 71-71
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    2. In Vitro Reconstitution of Microtubule Dynamics and Severing Imaged by Label-Free Interference-Reflection Microscopy

      • Yin-Wei Kuo, Jonathon Howard
      Pages 73-91

    3. Characterizing the Number of Kinesin Motors Bound to Microtubules in the Gliding Motility Assay Using FLIC Microscopy

      • Virginia VanDelinder, George D. Bachand
      Pages 93-104

    4. Design of Mechanical and Electrical Properties for Multidirectional Control of Microtubules

      • Hang Zhou, Taikopaul Kaneko, Naoto Isozaki, Ryuji Yokokawa
      Pages 105-119

    5. Linear-Zero Mode Waveguides for Single-Molecule Fluorescence Observation of Nucleotides in Kinesin-Microtubule Motility Assay

      • Kazuya Fujimoto, Ryota Iino, Ryuji Yokokawa
      Pages 121-131

    6. Microtubules and Quantum Dots Integration Leads to Conjugates with Applications in Biosensors and Bionanodevices

      • Xiao Hu, Cerasela Zoica Dinu
      Pages 133-148

  4. Microtubule-Based Active Matters

    1. Front Matter

      Pages 149-149
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    2. Assembling Microtubule-Based Active Matter

      • Alexandra M. Tayar, Linnea M. Lemma, Zvonimir Dogic
      Pages 151-183

    3. Spontaneous Alignment of Microtubules Via Tubulin Polymerization in a Narrow Space Under a Temperature Gradient

      • Kazuhiro Shikinaka
      Pages 185-192

    4. Dynamic Pattern Formation of Active Matters Triggered by Mechanical Stimuli

      • Jakia Jannat Keya, Arif Md. Rashedul Kabir, Mousumi Akter, Akira Kakugo
      Pages 193-203

    5. Spontaneously Beating Biomimetic Structures

      • Isabella Guido
      Pages 205-218

    6. Construction of Molecular Robots from Microtubules for Programmable Swarming

      • Jakia Jannat Keya, Mousumi Akter, Arif Md. Rashedul Kabir, Mst. Rubaya Rashid, Akira Kakugo
      Pages 219-230

    7. Fabrication of Artificial Muscle from Microtubules, Kinesins, and DNA Origami Nanostructures

      • Jakia Jannat Keya, Mousumi Akter, Arif Md. Rashedul Kabir, Satsuki Ishii, Akira Kakugo
      Pages 231-240

  5. Microtubule-Binding Molecules

    1. Front Matter

      Pages 241-241
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Editors and Affiliations

  • Dept Chemistry and Biotechnology, Tottori University, Tottori, Japan

    Hiroshi Inaba

Bibliographic Information

  • Book Title: Microtubules

  • Book Subtitle: Methods and Protocols

  • Editors: Hiroshi Inaba

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1983-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-1982-7Published: 28 April 2022

  • Softcover ISBN: 978-1-0716-1985-8Published: 28 April 2023

  • eBook ISBN: 978-1-0716-1983-4Published: 27 April 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 486

  • Number of Illustrations: 21 b/w illustrations, 110 illustrations in colour

  • Topics: Biomedical Engineering and Bioengineering

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