Abstract
Salivary glands are exocrine glands composed of several cell types, including the ductal, acinar, and basal/myoepithelial cells. They play important roles in maintaining oral homeostasis and health. During early murine development, the salivary glands, which arise as epithelial buds, are produced from primitive oral epithelia through an interaction between the oral epithelium and mesenchyme.
We recently reported that salivary gland organoids can be generated from mouse embryonic stem cells (ESCs). We recapitulated the process of embryonic salivary gland development using an organoid culture system. The mouse ESC-derived salivary gland organoids consisted of acinar-, ductal-, and myoepithelial-like cells. In this chapter, we describe a protocol for differentiating salivary gland organoids from ESCs .
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References
Atkinson JC, Grisius M, Massey W (2005) Salivary hypofunction and xerostomia: diagnosis and treatment. Dent Clin N Am 49:309–326
Guggenheimer J, Moore PA (2003) Xerostomia: etiology, recognition and treatment. J Am Dent Assoc 134:61–119
Rocchi C, Emmerson E (2020) Mouth-watering results: clinical need, current approaches, and future directions for salivary gland regeneration. Trends Mol Med 26(7):649–669. https://doi.org/10.1016/j.molmed.2020.03.009
Koehler KR, Mikosz AM, Molosh AI et al (2013) Generation of inner ear sensory epithelia from pluripotent stem cells in 3D culture. Nature 500:217–221
Eiraku M, Watanabe K, Matsuo-Takasaki M et al (2008) Self-organized formation of polarized cortical tissues from ESCs and its active manipulation by extrinsic signals. Cell Stem Cell 3:519–532
Suga H, Kadoshima T, Minaguchi M et al (2011) Self-formation of functional adenohypophysis in three-dimensional culture. Nature 480:57–62
Antonica F, Kasprzyk DF, Opitz R et al (2012) Generation of functional thyroid from embryonic stem cells. Nature 491:66–71
Lancaster MA, Knoblich JA (2014) Organogenesis in a dish: modeling development and disease using organoid technologies. Science 345:1247125
Eiraku M, Takata N, Ishibashi H et al (2011) Self-organizing optic-cup morphogenesis in three-dimensional culture. Nature 472:51–56
Spence JR, Mayhew CN, Rankin SA et al (2011) Directed differentiation of human pluripotent stem cells into intestinal tissue in vitro. Nature 470:105–109
Takebe T, Sekine K, Enomura M et al (2013) Vascularized and functional human liver from an iPSC-derived organ bud transplant. Nature 499:481–484
Taguchi A, Nishinakamura R (2017) Higher-order kidney organogenesis from pluripotent stem cells. Cell Stem Cell 21:730–746.e6
Takasato M, Er PX, Chiu HS et al (2016) Kidney organoids from human iPS cells contain multiple lineages and model human nephrogenesis. Nature 536:238
Ozone C, Suga H, Eiraku M et al (2016) Functional anterior pituitary generated in self-organizing culture of human embryonic stem cells. Nat Commun 7:1–10
Tanaka J, Ogawa M, Hojo H et al (2018) Generation of orthotopically functional salivary gland from embryonic stem cells. Nat Commun 9:4216
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Tanaka, J., Mishima, K. (2022). Generation of Salivary Gland Organoids from Mouse Embryonic Stem Cells. In: Kannan, N., Beer, P. (eds) Stem Cell Assays. Methods in Molecular Biology, vol 2429. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1979-7_16
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DOI: https://doi.org/10.1007/978-1-0716-1979-7_16
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-1978-0
Online ISBN: 978-1-0716-1979-7
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