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Generation of Embryonic Origin-Specific Vascular Smooth Muscle Cells from Human Induced Pluripotent Stem Cells

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Stem Cell Assays

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2429))

Abstract

Vascular smooth muscle cells (VSMCs), a highly mosaic tissue, arise from multiple distinct embryonic origins and populate different regions of our vascular network with defined boundaries. Accumulating evidence has revealed that the heterogeneity of VSMC origins contributes to region-specific vascular diseases such as atherosclerosis and aortic aneurysm. These findings highlight the necessity of taking into account lineage-dependent responses of VSMCs to common vascular risk factors when studying vascular diseases. This chapter describes a reproducible, stepwise protocol for the generation of isogenic VSMC subtypes originated from proepicardium, second heart field, cardiac neural crest, and ventral somite using human induced pluripotent stem cells. By leveraging this robust induction protocol, patient-derived VSMC subtypes of desired embryonic origins can be generated for disease modeling as well as drug screening and development for vasculopathies with regional susceptibility.

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Acknowledgments

This work was supported in part by research grants from NIH R01 HL1265276, R01 HL133272, R01 HL146690, R01 HL141371 (JCW), Tobacco-Related Disease Research Program (TRDRP) 30FT0852 (MS) and 27IR-0012 (JCW), and AHA 17MERIT33610009 (JCW) and AHA Career Development Award 19CDA34760019 (CL).

Author information

Authors and Affiliations

  1. Stanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA, USA

    Mengcheng Shen, Chun Liu & Joseph C. Wu

  2. Division of Cardiology, Department of Medicine, Stanford University School of Medicine, Stanford, CA, USA

    Joseph C. Wu

  3. Department of Radiology, Stanford University School of Medicine, Stanford, CA, USA

    Joseph C. Wu

Authors
  1. Mengcheng Shen
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  2. Chun Liu
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  3. Joseph C. Wu
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Corresponding author

Correspondence to Joseph C. Wu .

Editor information

Editors and Affiliations

  1. Stem Cell and Cancer Biology Laboratory, Division of Experimental Medicine and Pathology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN, USA

    Nagarajan Kannan

  2. Hull York Medical School, York, UK

    Philip Beer

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Shen, M., Liu, C., Wu, J.C. (2022). Generation of Embryonic Origin-Specific Vascular Smooth Muscle Cells from Human Induced Pluripotent Stem Cells. In: Kannan, N., Beer, P. (eds) Stem Cell Assays. Methods in Molecular Biology, vol 2429. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1979-7_15

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  • DOI: https://doi.org/10.1007/978-1-0716-1979-7_15

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1978-0

  • Online ISBN: 978-1-0716-1979-7

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