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Advancement of Single-Cell Sequencing in Medulloblastoma

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Medulloblastoma

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2423))

Abstract

Single-cell sequencing is a promising attempt to investigate the genomic, transcriptomic, and multiomic level of individual cell in the larger population of cells. The outward evolution of the technique from a manual method to the automation of single-cell sequencing is cogent. Lately, single-cell sequencing is widely used in various fields of science and has applications in neurobiology, immunity, cancer, microbiology, reproduction, and digestion. This chapter introduces the reader to the details of single-cell sequencing, currently used in several small-scale and commercial platforms. The advancement of single-cell sequencing in brain cancer sheds light on questions unanswered so far in the field of oncology.

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Abbreviations

CNV :

Copy number variation

FACS :

Fluorescence activated cell sorting

FISH:

Fluorescence in situ hybridization

MALBAC :

Multiple Annealing and Looping Based Amplification Cycles

MDA :

Multiple displacement amplification

NGS :

Next-generation sequencing

RT-PCR:

Reverse transcription Polymerase Chain Reaction

scRNA-seq:

Single-cell RNA sequencing

SCS :

Single-cell sequencing

WGA:

Whole genome amplification

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Authors and Affiliations

  1. School of Basic Sciences and BioX center, Indian Institute of Technology Mandi, Mandi, Himachal Pradesh, India

    Deepanshu Verma, Namyashree Nayak, Ashuthosh Singh & Ashutosh Kumar Singh

  2. Department of Medicinal Chemistry, Faculty of Ayurveda, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India

    Neha Garg

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  1. Deepanshu Verma
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  2. Namyashree Nayak
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  3. Ashuthosh Singh
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  4. Ashutosh Kumar Singh
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  5. Neha Garg
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Correspondence to Neha Garg .

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Editors and Affiliations

  1. Department of Pediatrics, Emory University, Atlanta, GA, USA

    Abhinav Dey

  2. Department of Pediatrics, Emory University, Atlanta, GA, USA

    Anshu Malhotra

  3. Department of Medicinal Chemistry, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India

    Neha Garg

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Verma, D., Nayak, N., Singh, A., Singh, A.K., Garg, N. (2022). Advancement of Single-Cell Sequencing in Medulloblastoma. In: Dey, A., Malhotra, A., Garg, N. (eds) Medulloblastoma. Methods in Molecular Biology, vol 2423. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1952-0_7

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  • DOI: https://doi.org/10.1007/978-1-0716-1952-0_7

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