Abstract
RNA interference (RNAi) is an evolutionarily conserved post-transcriptional gene silencing mechanism that responds to double-stranded RNA (dsRNA) by sequence-specific downregulation of target genes. The dsRNA-mediated RNAi technology has become one of the most widely used and powerful tools for functional genomic studies in diverse organisms. However, its application has been limited due to the technical difficulty of making RNAi constructs caused by the inverted repeat structure that is required for the formation of hairpin RNA. Here, we present a ligation-independent cloning-based dual vector-mediated RNAi system for silencing specific genes in plants. This approach is simple, efficient, and cost-effective and can be readily adapted to other binary vectors for functional analysis of target genes and the development of sustainable disease and pest control strategies in a broad range of plant species.
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Acknowledgments
This work was supported by a startup fund from the Texas A&M AgriLife Research and a Hatch Project from the USDA National Institute of Food and Agriculture to JS (TEX0-1-9675). We thank Dr. Yule Liu for providing the pYL41 and pRNAi-LIC vectors.
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Zhao, J., Rios, C.G., Xu, J., Ahmad, I., Song, J. (2022). Development of a Ligation-Independent Cloning-Based Dual Vector System for RNA Interference in Plants. In: Mysore, K.S., Senthil-Kumar, M. (eds) Plant Gene Silencing. Methods in Molecular Biology, vol 2408. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1875-2_18
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DOI: https://doi.org/10.1007/978-1-0716-1875-2_18
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