Abstract
Immunofluorescence (IF) on tissue sections allows for the detection of protein species subcellular localization. IF studies further offer the ability to achieve this understanding at the level of single cell granularity. Here, we describe the processes by which tissue is fixed, embedded, sectioned, and subsequently utilized for conducting indirect IF assays. We raise potential opportunities for troubleshooting and optimization at varying stages of the protocol.
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Aros, C.J. (2022). Indirect Immunofluorescence of Tissue Sections. In: Ooi, A.T. (eds) Single-Cell Protein Analysis. Methods in Molecular Biology, vol 2386. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1771-7_2
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DOI: https://doi.org/10.1007/978-1-0716-1771-7_2
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-1770-0
Online ISBN: 978-1-0716-1771-7
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