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Imaging of Actin Cytoskeleton in the Nematode Caenorhabditis elegans

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Cytoskeleton

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2364))

Abstract

The nematode Caenorhabditis elegans is one of the major model organisms in cell and developmental biology. This organism is easy to culture in laboratories and suitable for microscopic investigation of the cytoskeleton. Because the worms are small and transparent, the actin cytoskeleton in many tissues and cells can be observed with appropriate visualization techniques without sectioning or dissection. This chapter describes the introduction to representative methods for imaging the actin cytoskeleton in C. elegans and a protocol for staining worms with fluorescent phalloidin.

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Authors and Affiliations

  1. Departments of Pathology and Cell Biology, Emory University School of Medicine, Atlanta, GA, USA

    Shoichiro Ono

  2. Winship Cancer Institute, Emory University School of Medicine, Atlanta, GA, USA

    Shoichiro Ono

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  1. Shoichiro Ono
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Correspondence to Shoichiro Ono .

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Editors and Affiliations

  1. Professor Emeritus, Brooklyn College – CUNY, Brooklyn, NY, USA

    Ray H. Gavin

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Ono, S. (2022). Imaging of Actin Cytoskeleton in the Nematode Caenorhabditis elegans. In: Gavin, R.H. (eds) Cytoskeleton . Methods in Molecular Biology, vol 2364. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1661-1_7

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  • DOI: https://doi.org/10.1007/978-1-0716-1661-1_7

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1660-4

  • Online ISBN: 978-1-0716-1661-1

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