Abstract
Modification of plant mitochondrial genomes is still a difficult task, especially in multicellular plants. Transcription activator-like effector nucleases with a mitochondrial localization signal (mitoTALENs) can cut out a desired sequence from the mitochondrial genome in plants. Although vector construction of mitoTALENs is complicated, the modification efficiency is high enough to achieve homoplasmy of multicopy mitochondrial genomes. Here I describe how to design mitoTALENs to select a target, construct a vector, and select the mitochondrial transformants.
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Acknowledgments
This research was partly supported by grants from the Takeda Science Foundation, the Univ. of Tokyo GAP fund program, and partly from the Japan Society for the Promotion of Science (Grant Number 19H02927 and 19KK0391 to S. A.). We thank Mrs. Yoshiko Tamura for technical advice.
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Arimura, Si. (2022). MitoTALENs: A Method for Targeted Gene Disruption in Plant Mitochondrial Genomes. In: Van Aken, O., Rasmusson, A.G. (eds) Plant Mitochondria. Methods in Molecular Biology, vol 2363. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1653-6_22
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DOI: https://doi.org/10.1007/978-1-0716-1653-6_22
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