Abstract
The study of plant mitochondria often requires isolation of mitochondria from plant tissues in intact and functional form. Here, we describe an effective procedure of mitochondrial isolation from leaf tissues and whole seedlings of the model dicot species Arabidopsis thaliana by using differential centrifugation and continuous Percoll density gradients.
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Acknowledgments
OVA and HCT were supported by the Swedish Research Council (Vetenskapsrådet 2017-03854), Crafoord Foundation (20170862;20190868), Carl Tryggers Foundation (CTS 17: 487).
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Tran, H.C., Van Aken, O. (2022). Purification of Leaf Mitochondria from Arabidopsis thaliana Using Percoll Density Gradients. In: Van Aken, O., Rasmusson, A.G. (eds) Plant Mitochondria. Methods in Molecular Biology, vol 2363. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1653-6_1
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DOI: https://doi.org/10.1007/978-1-0716-1653-6_1
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