Skip to main content

Dual-Fluorescent Nanoparticle Probes Consisting of a Carbon Nanodot Core and a Molecularly Imprinted Polymer Shell

  • Protocol
  • First Online:
Molecularly Imprinted Polymers

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2359))

Abstract

Dual-fluorescent molecularly imprinted nanoparticles with a red-emissive carbon nanodot-doped silica core and a chlorogenic acid-imprinted fluorescent polymer layer are prepared and their use in ratiometric fluorometric analysis is described. Nanoparticle probes consisting of a shielded and stably emitting core and a shell with embedded binding sites that indicates the presence of an analyte with a change in emission allow for internally referenced measurements potentially accounting for detrimental influences from instrument drifts, light source fluctuations or sensor materials-related inhomogeneities.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 109.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 139.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Ahmad OS, Bedwell TS, Esen C et al (2019) Molecularly imprinted polymers in electrochemical and optical sensors. Trends Biotechnol 37:294–309

    Article  CAS  Google Scholar 

  2. Turiel E, Martin-Esteban A (2010) Molecularly imprinted polymers for sample preparation: a review. Anal Chim Acta 668:87–99

    Article  CAS  Google Scholar 

  3. Mayes AG, Whitcombe MJ (2005) Synthetic strategies for the generation of molecularly imprinted organic polymers. Adv Drug Deliv Rev 57:1742–1778

    Article  CAS  Google Scholar 

  4. Liu G, Huang X, Li L et al (2019) Recent advances and perspectives of molecularly imprinted polymer-based fluorescent sensors in food and environment analysis. Nanomaterials 9:1030

    Article  CAS  Google Scholar 

  5. Rico-Yuste A, Carrasco S (2019) Molecularly imprinted polymer-based hybrid materials for the development of optical sensors. Polymers 11:1173

    Article  Google Scholar 

  6. Wan W, Wagner S, Rurack K (2016) Fluorescent monomers: “bricks” that make a molecularly imprinted polymer “bright”. Anal Bioanal Chem 408:1753–1771

    Article  CAS  Google Scholar 

  7. Inoue Y, Kuwahara A, Ohmori K et al (2013) Fluorescent molecularly imprinted polymer thin films for specific protein detection prepared with dansyl ethylenediamine-conjugated O-acryloyl L-hydroxyproline. Biosens Bioelectron 48:113–119

    Article  CAS  Google Scholar 

  8. Zhang X, Yang S, Jiang R et al (2018) Fluorescent molecularly imprinted membranes as biosensor for the detection of target protein. Sens Actuators B-Chem 254:1078–1086

    Article  CAS  Google Scholar 

  9. Guney O, Cebeci FC (2010) Molecularly imprinted fluorescent polymers as chemosensors for the detection of mercury ions in aqueous media. J Appl Polym Sci 117:2373–2379

    Article  Google Scholar 

  10. Chen L, Liu D, Peng J et al (2019) Ratiometric fluorescence sensing of metal-organic frameworks: tactics and perspectives. Coord Chem Rev 404:213113

    Article  Google Scholar 

  11. Wu P, Hou X, Xu JJ et al (2016) Ratiometric fluorescence, electrochemiluminescence, and photoelectrochemical chemo/biosensing based on semiconductor quantum dots. Nanoscale 8:8427–8442

    Article  CAS  Google Scholar 

  12. Zhang R, Zhang Y, Deng X et al (2018) A novel dual-signal electrochemical sensor for bisphenol A determination by coupling nanoporous gold leaf and self-assembled cyclodextrin. Electrochim Acta 271:417–424

    Article  CAS  Google Scholar 

  13. Baker SN, Baker GA (2010) Luminescent carbon nanodots: emergent nanolights. Angew Chem Int Ed 49:6726–6744

    Article  CAS  Google Scholar 

  14. Hola K, Zhang Y, Wang Y et al (2014) Carbon dots-emerging light emitters for bioimaging, cancer therapy and optoelectronics. Nano Today 9:590–603

    Article  CAS  Google Scholar 

  15. Clifford MN, Jaganath IB, Ludwig IA et al (2017) Chlorogenic acids and the acyl-quinic acids: discovery, biosynthesis, bioavailability and bioactivity. Nat Prod Rep 34:1391–1421

    Article  CAS  Google Scholar 

  16. Wan W, Biyikal M, Wagner R et al (2013) Fluorescent sensory microparticles that “light-up” consisting of a silica core and a molecularly imprinted polymer (MIP) shell. Angew Chem Int Ed 52:7023–7027

    Article  CAS  Google Scholar 

  17. Shinde S, El-Schich Z, Malakpour A et al (2015) Sialic acid-imprinted fluorescent core-shell particles for selective labeling of cell surface glycans. J Am Chem Soc 137:13908–13912

    Article  CAS  Google Scholar 

  18. ImageJ Basics. National Institutes of Health (NIH). https://imagej.nih.gov/ij/docs/pdfs/ImageJ.pdf. Accessed 15 Sep 2020

  19. Pan LL, Sun S, Zhang L et al (2016) Near-infrared emissive carbon dots for two-photon fluorescence bioimaging. Nanoscale 8:17350–17356

    Article  CAS  Google Scholar 

  20. Santra S, Bagwe RP, Dutta D et al (2005) Synthesis and characterization of fluorescent, radio-opaque, and paramagnetic silica nanoparticles for multimodal bioimaging applications. Adv Mater 17:2165–2169

    Article  CAS  Google Scholar 

  21. Bagwe RP, Yang CY, Hilliard LR et al (2004) Optimization of dye-doped silica nanoparticles prepared using a reverse microemulsion method. Langmuir 20:8336–8342

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This work has received funding from the European Union’s Horizon 2020 research and innovation program under the Marie Skłodowska-Curie grant agreement No 721297.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Knut Rurack .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2021 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

About this protocol

Check for updates. Verify currency and authenticity via CrossMark

Cite this protocol

Jiang, S., Gawlitza, K., Rurack, K. (2021). Dual-Fluorescent Nanoparticle Probes Consisting of a Carbon Nanodot Core and a Molecularly Imprinted Polymer Shell. In: Martín-Esteban, A. (eds) Molecularly Imprinted Polymers. Methods in Molecular Biology, vol 2359. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1629-1_17

Download citation

  • DOI: https://doi.org/10.1007/978-1-0716-1629-1_17

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1628-4

  • Online ISBN: 978-1-0716-1629-1

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics