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Comprehensive Phytotoxicity Assessment Protocol for Engineered Nanomaterials

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Environmental Toxicology and Toxicogenomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2326))

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Abstract

In order for nanotechnology to be sustainably applied in agriculture, emphasis should be on comprehensive assessment of multiple endpoints, including biouptake and localization of engineered nanomaterials (ENMs), potential effects on food nutrient quality, oxidative stress responses, and crop yield, before ENMs are routinely applied in consumer and agronomic products. This chapter succinctly outlines a protocol for conducting nanophytotoxicity studies focusing on nanoparticle purification and characterization, arbuscular mycorrhizal fungi (AMF)/symbiont inoculation, biouptake and translocation/localization, varied endpoints of oxidative stress responses, and crop yield.

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Acknowledgements

LRP gratefully acknowledges funding support from East Carolina University (grant #111101 to LRP). CSU thanks ECU Graduate School and Department of Public Health for the graduate assistantship. SF would also like to thank Shahrekord University for the financial support (grant #96-GRN1M731 to SF).

Author information

Authors and Affiliations

  1. Department of Public Health, The Brody School of Medicine, East Carolina University, Greenville, NC, USA

    Lok R. Pokhrel & Chukwudi S. Ubah

  2. Department of Health Education and Promotion, College of Health and Human Performance, East Carolina University, Greenville, NC, USA

    Lok R. Pokhrel

  3. Department of Agronomy, Faculty of Agriculture, Shahrekord University, Shahrekord, Iran

    Sina Fallah

Authors
  1. Lok R. Pokhrel
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  2. Chukwudi S. Ubah
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  3. Sina Fallah
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Corresponding author

Correspondence to Lok R. Pokhrel .

Editor information

Editors and Affiliations

  1. Department of Biology, East Carolina University, Greenville, NC, USA

    Xiaoping Pan

  2. Department of Biology, East Carolina University, Greenville, NC, USA

    Baohong Zhang

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Pokhrel, L.R., Ubah, C.S., Fallah, S. (2021). Comprehensive Phytotoxicity Assessment Protocol for Engineered Nanomaterials. In: Pan, X., Zhang, B. (eds) Environmental Toxicology and Toxicogenomics. Methods in Molecular Biology, vol 2326. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1514-0_18

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  • DOI: https://doi.org/10.1007/978-1-0716-1514-0_18

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1513-3

  • Online ISBN: 978-1-0716-1514-0

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